Document Detail


Variable isoflavone content of red clover products affects intestinal disposition of biochanin A, formononetin, genistein, and daidzein.
MedLine Citation:
PMID:  18370585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Marketed red clover (Trifoleum pratense) products use a wide variety of labels, and the isoflavone content from the label is ambiguous.
MATERIALS AND METHODS: In the present study, we analyzed the content of various isoflavone products, and determined (1) the content and (2) how the sample matrix of red clover products affects the intestinal disposition of main isoflavones within it, using the human intestinal Caco-2 cell model.
RESULTS: Analysis using high- and ultraperformance liquid chromatography indicates that the isoflavone content varied significantly (p < 0.05) between the chosen products. Consequently, rates of isoflavone absorption across the Caco-2 cell monolayers varied (p < 0.05) greatly. Unexpectedly, permeabilities of biochanin A and formononetin (two key biomarkers) were found to be significantly affected (p < 0.05) by the product matrix. As expected, biochanin A was the only isoflavone with noticeable metabolite peaks in both the apical and basolateral sides. Interestingly, rates of metabolism and the polarity of the glucuronidated biochanin A excretion were also significantly altered (p < 0.05) by the product matrix. Studies using the breast cancer resistance protein (BCRP) inhibitor, dipyridamole, showed that both the apical and basolateral excretion of biochanin A glucuronides were significantly (p < 0.05) reduced (7.5- and 9.4-fold, respectively) when dipyridamole is present. This provides evidence that BCRP is the main transporter responsible for the apical efflux of isoflavone glucuronides.
CONCLUSIONS: The isoflavone content of the marketed red clover products is highly variable, and the product matrix significantly affected the intestinal disposition of red clover isoflavones by altering their absorption rates, permeabilities, biochanin A glucuronide excretion rates, and the polarity of biochanin A glucuronide excretion. This research provides scientific evidence to support the standardization effort, so that consumers can make intelligent product choices.
Authors:
Stephen W J Wang; Yan Chen; Tiby Joseph; Ming Hu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of alternative and complementary medicine (New York, N.Y.)     Volume:  14     ISSN:  1075-5535     ISO Abbreviation:  J Altern Complement Med     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-10     Completed Date:  2008-07-11     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9508124     Medline TA:  J Altern Complement Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  287-97     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Caco-2 Cells / metabolism
Chromatography, High Pressure Liquid
Genistein / pharmacokinetics*
Humans
Intestinal Absorption / physiology*
Isoflavones / analysis*,  pharmacokinetics,  pharmacology*
Trifolium / chemistry*
Grant Support
ID/Acronym/Agency:
CA 87779/CA/NCI NIH HHS; GM70737/GM/NIGMS NIH HHS; R01 GM070737/GM/NIGMS NIH HHS; R01 GM070737-02/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Isoflavones; 295DQC67BJ/formononetin; 6287WC5J2L/daidzein; DH2M523P0H/Genistein; U13J6U390T/biochanin A
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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