Document Detail


Variable degradation of factor VIII-related protein in lyophilised concentrates of antihaemophilic factor (AHF).
MedLine Citation:
PMID:  664567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Factor VIII-related properties (coagulant = VIII : C, 'Willebrand' factor = VIII R : WF, antigen = VIII R : AG) are measured in a constant proportion in normal plasma and certain preparations of highly purified factor VIII (relative ratios: 0.5--1.5). We tested these activities in some commercial, lyophilised concentrates of factor VIII and found a variable increase of the ratio VIII R : AG/VIII R : WF. The relative increase of VIII R : AG, and/or loss of VIII R : WF, was attributed to variable degradation of factor VIII-related protein(s) which was directly visualized by electrophoresis on 2.75% polyacrylamide gels in the presence of sodium dodecyl sulphate.
Authors:
T Jakab; R Pflugshaupt; M Furlan; E A Beck
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Vox sanguinis     Volume:  35     ISSN:  0042-9007     ISO Abbreviation:  Vox Sang.     Publication Date:    1978 Jul-Aug
Date Detail:
Created Date:  1978-08-28     Completed Date:  1978-08-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0413606     Medline TA:  Vox Sang     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  36-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens / isolation & purification
Electrophoresis, Polyacrylamide Gel
Factor VIII / immunology,  isolation & purification,  metabolism*
Freeze Drying
Humans
Chemical
Reg. No./Substance:
0/Antigens; 9001-27-8/Factor VIII

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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