| Variable Phase Dark-Field Contrast-A Variant Illumination Technique for Improved Visualizations of Transparent Specimens. | |
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MedLine Citation:
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PMID: 22380758 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Variable phase dark-field contrast has been developed as an illumination technique in light microscopy, which promises significant improvements and a higher variability in imaging of several transparent specimens. In this method, a phase contrast image is optically superimposed on a dark-field image so that a partial image based on the principal zeroth-order maximum (phase contrast) interferes with an image that is based on the secondary maxima (dark field). The background brightness and character of the resulting image can be continuously modulated from a phase-contrast-dominated to a dark-field-dominated character. The condenser aperture diaphragm can be used for modulations of the image's appearance. Specimens can either be illuminated concentrically or obliquely (eccentrically) when parts of the illuminating light beams are covered and blocked. Moreover, a bright-field-like partial image can be added. In this way, the illumination can be optimally adjusted to the specific properties of the specimen. The techniques described can lead to improved visual information especially in biological specimens consisting of phase structures and additional light-absorbing or -reflecting components. Moreover, the specimen's three-dimensionality can be accentuated with improved clarity because the illuminating light beams associated with phase contrast and dark field run to the specimen at different angles of incidence. |
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Authors:
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Timm Piper; Jörg Piper |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-2 |
Journal Detail:
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Title: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada Volume: - ISSN: 1435-8115 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9712707 Medline TA: Microsc Microanal Country: - |
Other Details:
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Languages: ENG Pagination: 1-10 Citation Subset: - |
Affiliation:
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Laboratory for Applied Microscopy Research-Light Microscopy, Marienburgstr. 23, Bullay, RLP-56859, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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