Document Detail


Variability of triacylglycerol content in human skeletal muscle biopsy samples.
MedLine Citation:
PMID:  8889747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The variability of the triacylglycerol store in human skeletal muscle (TGm) was examined using the needle biopsy technique. In 13 subjects, three biopsies were sampled from the vastus lateralis muscle of one leg at rest and after 90 min of cycling at 65% of maximal O2 uptake on one or two occasions. Visible fat and blood were removed before the samples were frozen, and remaining blood, connective tissue, and fat were removed from freeze-dried fiber bundles. TGm content was measured in two aliquots of powdered muscle from each biopsy. Within-biopsy variability was low at 6%. Despite precautions, many biopsies from inactive subjects were contaminated with adipose tissue. The TGm between-biopsy coefficient of variation (CV) was 23.5 +/- 14.6% (SD, n = 24) for rest and exercise time points where three noncontaminated biopsies existed. The between-biopsy variability at rest (19.8 +/- 7.9%, n = 10) was not significantly different from that at exercise (26.1 +/- 17.4%, n = 14). The muscle glycogen between-biopsy CV for rest and exercise time points was 10.0 +/- 10.3%. The resting TGm content was 26.3 +/- 4.3 mmol/kg dry muscle, and the net utilization during the 90 min of exercise was less than the between-biopsy variability. It is concluded that the TGm store measured in repeated biopsies of human skeletal muscle is variable, with a CV of 20-26%. Therefore, because of this high variability, only changes greater than approximately 24% of resting TGm content may be considered meaningful.
Authors:
P S Wendling; S J Peters; G J Heigenhauser; L L Spriet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  81     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-07     Completed Date:  1997-02-07     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1150-5     Citation Subset:  IM; S    
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biopsy
Exercise / physiology*
Female
Humans
Male
Muscle, Skeletal / metabolism*
Triglycerides / metabolism*
Chemical
Reg. No./Substance:
0/Triglycerides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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