Document Detail

Variability of sputum inflammatory mediators in COPD and α1-antitrypsin deficiency.
MedLine Citation:
PMID:  22700846     Owner:  NLM     Status:  MEDLINE    
There is inherent daily variability of sputum inflammatory mediators in stable-state patients with usual chronic obstructive pulmonary disease (COPD). The variability of pulmonary inflammation in patients with α(1)-antitrypsin deficiency (A1ATD) is unknown. Our study aimed to quantify this variability, in comparison to patients with usual COPD, in order to facilitate power calculations for proof of concept trials of putative specific anti-inflammatory agents in both groups. Sputum interleukin (IL)-8, myeloperoxidase (MPO), leukotriene B(4) (LTB(4)) and differential cell counts were measured in 12 usual COPD patients and 12 A1ATD patients on nine occasions over a 1-month period. All samples were obtained in the stable clinical state. There was significant daily variability in all mediators in all patients. A1ATD patients had higher sputum concentrations of IL-8 and LTB(4) compared with usual COPD, but lower levels of MPO and absolute neutrophil counts. Patients with usual COPD had more intra-patient variability, A1ATD patients demonstrated greater inter-patient variability. There are increased concentrations of pulmonary inflammatory mediators but fewer sputum neutrophils in A1ATD compared with usual COPD. The daily variability of inflammatory mediators and cell counts was significantly reduced in both groups by averaging sequential samples. This can be utilised to perform power calculations for future proof of concept studies; averaging three sequential samples appears optimum.
Helen Stone; Gillian McNab; Alice M Wood; Robert A Stockley; Elizabeth Sapey
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Publication Detail:
Type:  Journal Article     Date:  2012-06-14
Journal Detail:
Title:  The European respiratory journal     Volume:  40     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-03     Completed Date:  2013-02-04     Revised Date:  2013-05-23    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  561-9     Citation Subset:  IM    
School of Clinical and Experimental Medicine, University of Birmingham, Birmingham, B15 2TT, UK.
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MeSH Terms
Cell Count
Inflammation Mediators / analysis,  metabolism*
Interleukin-8 / analysis
Leukotriene B4 / analysis
Middle Aged
Peroxidase / analysis
Pulmonary Disease, Chronic Obstructive / metabolism*
Sputum / chemistry*
alpha 1-Antitrypsin Deficiency / metabolism*
Reg. No./Substance:
0/IL8 protein, human; 0/Inflammation Mediators; 0/Interleukin-8; 71160-24-2/Leukotriene B4; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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