Document Detail


Variability of protein and phosphoprotein levels in clinical tissue specimens during the pre-analytical phase.
MedLine Citation:
PMID:  23134551     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The quality of human tissue specimens can have a significant impact on analytical data sets for biomarker research. The aim of this study was to characterise fluctuations of protein and phosphoprotein levels in human tissue samples during the pre-analytical phase. Eleven intestine and 17 liver specimens were surgically resected, aliquoted and either snap frozen or fixed in formalin immediately or exposed to different ischemic conditions before preservation. Protein levels in the resultant samples were investigated by reverse phase protein array, western blot analysis and liquid chromatography-tandem mass spectrometry. Our data revealed that the degree of sensitivity of proteins and phosphoproteins to delayed preservation varied between different patients and tissue types. For example, up-regulation of phospho-p42/44 MAPK in intestine samples was seen in some patients but not in others. General trends towards up- or down-regulation of most proteins were not evident due to pronounced inter-patient variability but signal intensities of only a few proteins, such as cytokeratin 18, were altered from baseline in post-resection samples. In contrast, glyceraldehyde 3-phosphate dehydrogenase was found to be stable during periods of cold ischemia. Our study represents a proper approach for studying potential protein fluctuations in tissue specimens for future biomarker development programs.
Authors:
Sibylle Gündisch; Stefanie M Hauck; Hakan Sarioglu; Christina Schott; Christian Viertler; Marcel Kap; Tibor Schuster; Bilge Reischauer; Robert Rosenberg; Cornelis Verhoef; Hans-Joerg Mischinger; Peter Riegman; Kurt Zatloukal; Karl-Friedrich Becker
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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