| Variability in benthic diazotrophy and cyanobacterial diversity in a tropical intertidal lagoon. | |
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MedLine Citation:
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PMID: 18199084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Benthic nitrogen fixation has been estimated to contribute 15 Tg N year(-1) to the marine nitrogen budget. With benthic marine nitrogen fixation being largely overlooked in more recent surveys, a refocus on benthic diazotrophy was considered important. Variations in nitrogenase activity (acetylene reduction-gas chromatography) in a tropical lagoon in the western Indian Ocean (Zanzibar, Tanzania) were monitored over a 3-year period (2003-2005) and related to cyanobacterial and diazotrophic microbial diversity using a polyphasic approach. Different nitrogenase activity patterns were discerned, with the predominant pattern being high daytime activities combined with low nighttime activities. Analyses of the morphological and 16S rRNA gene diversity among cyanobacteria revealed filamentous nonheterocystous (Oscillatoriales) and unicellular (Chroococcales) representatives to be predominant. Analyses of the nifH gene diversity showed that the major phylotypes belonged to noncyanobacterial prokaryotes. However, as shown by cyanobacterial selective nifH-denaturing gradient gel electrophoresis analysis, cyanobacterial nifH gene sequences were present at all sites. Several nifH and 16S rRNA gene phylotypes were related to uncultured cyanobacteria or bacteria of geographically distant habitats, stressing the widespread occurrence of still poorly characterized microorganisms in tropical benthic marine communities. |
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Authors:
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Karolina Bauer; Beatriz Díez; Charles Lugomela; Susanna Seppälä; Agneta Julia Borg; Birgitta Bergman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEMS microbiology ecology Volume: 63 ISSN: 0168-6496 ISO Abbreviation: FEMS Microbiol. Ecol. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-01-17 Completed Date: 2008-03-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8901229 Medline TA: FEMS Microbiol Ecol Country: England |
Other Details:
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Languages: eng Pagination: 205-21 Citation Subset: IM |
Affiliation:
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Department of Botany, Stockholm University, Stockholm, Sweden. bauer@botan.su.se |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EF160007; EF160008; EF160009; EF160010; EF160011; EF160012; EF160013; EF160014; EF160015; EF160016; EF160017; EF160018; EF160019; EF160020; EF160021; EF160022; EF160023; EF160024; EF160033; EF160034; EF160035; EF160036; EF160037; EF160038; EF160039; EF160040; EF160041; EF160042; EF160043; EF160044; EF160045; EF160046; EF160047; EF160048; EF160049; EF160050; EF160051; EF160052; EF160053; EF160054; EF160055; EF160056; EF160057; EF160058; EF160059; EF160060; EF160061; EF160062; EF160063; EF160064 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Biodiversity Cloning, Molecular Cyanobacteria / classification, genetics, isolation & purification*, ultrastructure Microscopy, Electron, Scanning Molecular Sequence Data Nitrogenase / metabolism* Oxidoreductases / genetics* Phylogeny Polymorphism, Restriction Fragment Length RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, RNA Tanzania Tropical Climate |
| Chemical | |
Reg. No./Substance:
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0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; EC 1.-/Oxidoreductases; EC 1.18.6.1/Nitrogenase; EC 1.18.6.1/nitrogenase reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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