Document Detail


Variability of Bartonella genotypes among small mammals in Spain.
MedLine Citation:
PMID:  20935117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to study which Bartonella genotypes are circulating among small mammals in Spain, we analyzed the spleens of 395 animals from three different areas-247 animals from the Basque Country (northern Spain), 121 animals from Catalonia (northeastern Spain), and 27 animals from Madrid (central Spain)-by a triplex PCR combined with a reverse line blot previously described by our group. The prevalence of Bartonella was 26.8% (106/395), and in 4.8% (19/395) of the animals more than one Bartonella genotype was detected. The study of gltA and the intergenic transcribed spacer in the positive samples demonstrated a large diversity, allowing the assignation of them into 22 genotypes. The most prevalent genotypes were 2 and 3, which are closely related to Bartonella taylorii. In addition, nine genotypes were associated with specific mammal species. Genotypes close to the zoonotic Bartonella grahamii, Bartonella elizabethae, and Bartonella rochalimae were also detected. Ten genotypes showed a percentage of similarity with known Bartonella species lower than 96%, suggesting the presence of potential new species. Further studies of the impact of these pathogens on human health and especially in cases of febrile illness in Spain are strongly recommended. Furthermore, our method has been updated with 21 new probes in a final panel of 36, which represents a robust molecular tool for clinical and environmental Bartonella studies.
Authors:
H Gil; C García-Esteban; J F Barandika; J Peig; A Toledo; R Escudero; I Jado; M Rodríguez-Vargas; C García-Amil; B Lobo; P Roales; I Rodríguez-Moreno; A S Olmeda; A L García-Pérez; P Anda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-08
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-03     Completed Date:  2011-03-14     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8062-70     Citation Subset:  IM    
Affiliation:
Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,1 Hospital de Getafe, Getafe, Madrid, Spain. hgil@isciii.es
Data Bank Information
Bank Name/Acc. No.:
GENBANK/HM596431;  HM596432;  HM596433;  HM596434;  HM596435;  HM596436;  HM596437;  HM596438;  HM596439;  HM596440;  HM596441;  HM596442;  HM596443;  HM596444;  HM596445;  HM596446;  HM596447;  HM596448;  HM596449;  HM596450;  HM596451;  HM596452;  HM596453;  HM596454;  HM596455;  HM596456;  HM596457;  HM596458;  HM596459;  HM596460;  HM596461;  HM596462;  HM596463;  HM596464;  HM596465;  HM596466;  HM596467;  HM596468;  HM596469;  HM596470
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / genetics
Bartonella / classification*,  genetics*,  isolation & purification
Bartonella Infections / epidemiology,  veterinary*
Cluster Analysis
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal Spacer / chemistry,  genetics
Genetic Variation*
Genotype
Glutamate Synthase / genetics
Liver / microbiology
Mammals / microbiology*
Molecular Sequence Data
Phylogeny
Prevalence
Sequence Analysis, DNA
Spain / epidemiology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/DNA, Ribosomal Spacer; EC 1.4.1.13/Glutamate Synthase
Comments/Corrections

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