Document Detail

Vanishing testes: a literature review.
Jump to Full Text
MedLine Citation:
PMID:  22985611     Owner:  NLM     Status:  MEDLINE    
Vanishing testes syndrome is often referred to as testicular regression syndrome (TRS) in the recent medical literature. The most characteristic histological findings are presence of a fibrovascular nodule with associated hemosiderin-laden macrophages and dystrophic calcification. Residual testicular tubules are found in less than 10% of cases, with prevalence being unrelated to age at surgery. Presence of seminiferous tubules and viable germ cells in testicular remnant tissue has been reported in some series. TRS theoretically carries a potential for malignant degeneration in the long term and therefore removal of any remnant is a common practice to eliminate this risk. However, no case series has reported germinal dysplasia or intratubular germ cell neoplasia in any of the specimens taken from these patients.
Özgür Pirgon; Bumin Nuri Dündar
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Journal of clinical research in pediatric endocrinology     Volume:  4     ISSN:  1308-5735     ISO Abbreviation:  J Clin Res Pediatr Endocrinol     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-02-12     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101519456     Medline TA:  J Clin Res Pediatr Endocrinol     Country:  Turkey    
Other Details:
Languages:  eng     Pagination:  116-20     Citation Subset:  IM    
Süleyman Demirel University Faculty of Medicine, Department of Pediatric Endocrinology and Diabetes, Isparta, Turkey.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Gonadal Dysgenesis, 46,XY / diagnosis,  epidemiology,  physiopathology*,  therapy
Testis / abnormalities,  physiopathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Clin Res Pediatr Endocrinol
Journal ID (iso-abbrev): J Clin Res Pediatr Endocrinol
Journal ID (publisher-id): JCRPE
ISSN: 1308-5727
ISSN: 1308-5735
Publisher: Galenos Publishing
Article Information
Download PDF
© Journal of Clinical Research in Pediatric Endocrinology, Published by Galenos Publishing.
Received Day: 4 Month: 7 Year: 2012
Accepted Day: 16 Month: 7 Year: 2012
Print publication date: Month: 9 Year: 2012
Electronic publication date: Day: 11 Month: 9 Year: 2012
Volume: 4 Issue: 3
First Page: 116 Last Page: 120
ID: 3459158
PubMed Id: 22985611
DOI: 10.4274/Jcrpe.728
Publisher Item Identifier: 204

Vanishing Testes: A Literature Review
Özgur Pirgon1*
Bumin Nuri Dündar2
1 Süleyman Demirel University Faculty of Medicine, Department of Pediatric Endocrinology and Diabetes, Isparta, Turkey
2 Katip Çelebi University Faculty of Medicine, Department of Pediatric Endocrinology and Diabetes, Izmir, Turkey
Correspondence: * Address for Correspondence :. Phone : +90 246 211 93 02 E-mail :


Testicular regression syndrome (TRS) or ‘vanishing testis’ is acondition which is considered to be due to the subsequentatrophy and disappearance in fetal life of an initially normal testis(1). The presence of spermatic cord structures is evidence of thepresence of the testis in early intrauterine life. When associatedwith a blind-ending spermatic cord, this entity is named as the‘‘vanishing testis syndrome’’ in the urology literature (2) or TRSin the pathology literature (3), since anatomically it ischaracterized by a rudimentary spermatic cord with absence ofmacroscopically identifiable testicular tissue. This absence of atestis in an otherwise normal 46XY male is usually unilateral andis assumed to be a consequence of intrauterine or perinataltorsion or infarction.


TRS or vanishing testis syndrome may be seen in less than 5% of cryptorchidism cases (4). Cryptorchidism is not an uncommon condition, with about 3% of full-term neonates having an incompletely descended testis at birth - a prevalence decreasing to about 1% by the age of 1 year (5). The testis is nonpalpable in 10% to 20% of cryptorchidism cases, and of these, TRS accounts for 35% to 60% (6,7,8,9,10).

Absence of testis, intra-abdominal testis, inguinal or intra-abdominal vanishing testis are reasons for nonpalpable testis (11,12). Vanishing testis is more common than testicular agenesis in patients with nonpalpable testis (13). It is assumed that 40% of this subpopulation harbors a vanished testis, thus as many as 1 male per 1250 may be affected (14). An 8-year review of cases of nonpalpable testes at the Children’s Hospital of Philadelphia showed that 41% of 447 affected boys (181 patients) had an atrophic remnants or absent testis (15). Therefore, it seems that this syndrome is a common phenomenon; however, the optimal management of this condition remains unclear.

Lou and co-workers reported a series of patients with nonpalpable testes, of which 64% had cryptorchidism. Of these, 22.5% had complete absence of testis, vas deferens and epididymis, 15% had only a blindly-ending vas deferens, and 67.5% had a blindly-ending vas deferens with blood vessels in the inguinal canal (16).


Hormonal and mechanical factors have been implicated in the descent of the testes, but a specific factor has not yet been identified for the pathogenesis of undescended or nonpalpable testis. Although the etiology, pathophysiology, and prognosis of TRS and that of the viable undescended testis might be different, TRS is thought to be the result of late antenatal or perinatal vascular thrombosis, torsion, or endocrinopathy (6,17,18,19). Recent studies strongly support a vascular accident and antenatal torsion theory rather than an endocrinopathy. The incompletely descended testis is thought to be more prone to torsion during the fetal and perinatal period (1). This hypothesis is supported by the presence of hemosiderin-laden macrophages in surgically removed specimens (20), consistent with the venous congestion and hemorrhagic infarction secondary to torsion of a structure. On the other hand, trauma to the intrascrotal testis perinatally has been suggested as a risk factor (21). Earlier descent of the left testis may account for the side predilection. Of interest, the oldest patient in the literature was a 26-year-old man who presented with markedly small gonad 11 years after orchidopexy for cryptorchidism and this was removed as an atrophic remnant (4).

Histopathological comparison of the contralateral testis from patients with unilateral vanishing testis and undescended testis was performed by Huff et al (22) and they showed that the patients with an absent testis did not exhibit abnormal, lower germ cell counts suggestive of an endocrinopathic condition.

Testicular regression has been observed in association with genetic abnormality such as microdeletion of the Y chromosome and boys with persistence of Mullerian duct structures have been found to undergo TRS after birth (23). Additionally, early loss of both testes in the antenatal period can result in genital ambiguity. However, an association between genetic factors necessary for normal testicular descent and anorchia/vanishing testis syndrome has not been established (24).

In the vast majority of cases, TRS appears to be a sporadic occurrence, and the patients are otherwise normal with no significant family history. However, there are now several reports of TRS either in association with other defects, including severe mental retardation in chromosomally normal siblings, or occurring in several members of the same family, suggesting a possible genetic basis for the condition in some subjects (25,26).


The clinical manifestations of testicular regression can be associated with the embryological sequence and can be classified into early and late embryonic, and early, mid and late fetal or early neonatal events (18). This disorder may be unilateral or, less commonly, bilateral, with partial or complete absence of testicular tissue and, in the majority of cases, with normal external genitalia (Figure 1).

TRS patients usually exhibit a male phenotype. However, although uncommon, the phenotypic spectrum associated with TRS may vary from normal male with unilateral nonpalpable testis through phenotypic male with micropenis, to phenotypic female (27). The phenotype probably depends on the extent and timing of the intrauterine accident in relation to sexual development. These genetically male individuals (46, XY) present with unilateral or bilateral absence of detectable testis structures and Mullerian duct system (3,28,29) (Figure 2).

Embryonic TRS has been considered to be part of the clinical spectrum of partial 46, XY gonadal dysgenesis (30). Most of the patients with TRS exhibit features of intersex or severe micropenis in association with complete uni- or bilateral regression of testicular tissue. The degree of masculinization of the internal and external genitalia depends on the duration of testicular function prior to its loss. Variable degrees of sexual ambiguity have been reported in familial cases, but the nature of the underlying defect is still not clear. Recently, a novel heterozygous missense mutation (V355M) in SF1 gene was found in one boy with a micropenis and TRS (31).


Clinically, a unilateral nonpalpable testis may be associated with TRS, cryptorchidism, retractile testis, or testicular agenesis. Laparoscopy is widely used to distinguish among these conditions while avoiding open abdominal surgery. When, in the setting of a nonpalpable testicle, vas deferens and spermatic vessels are visualized exiting the internal inguinal ring on laparoscopy, a groin exploration may be carried out to identify and remove the testicular tissue remnant associated with a vanished testis (32).

Diagnosis of TRS is based on a clinically nonpalpable testis with a blind-ending spermatic cord located within the retroperitoneum or exiting a closed internal inguinal ring. Usually, a small fibrotic nodule is found at the end of this spermatic cord. Fibrosis, dystrophic calcification, and hemosiderin deposition in association with identifiable testicular/paratesticular structures are also features of TRS (33).

A number of criteria have been proposed to ascertain the diagnosis of the vanishing testis syndrome. These criteria are: 1) no testis palpated during an examination under anesthesia, 2) visualizing blind-ending spermatic vessels within the retroperitoneum or visualizing spermatic vessels and vas deferens exiting a closed internal inguinal ring.


In gross descriptions of the specimens, a spermatic cord with a small mass of firm, fibrotic tissue at one end, as well as elements of the vas deferens, spermatic artery and venous plexuses have been reported.

Smith et al (20) in their study have observed that histologically, the distal expansion of most of the specimens was composed of dense fibrovascular tissue in the absence of seminiferous tubules or normal testicular elements; scattered foci of calcification and brown pigment were present. The finding of dystrophic calcification and hemosiderin deposition, in the absence of viable testicular tissue but in the presence of relatively normal spermatic cord elements, supports the hypothesis of remote infarction leading to unilateral and occasionally bilateral anorchia. The young age of the patients and the history of an absent testis from birth are supportive of in utero accident. On the other hand, the above-mentioned histopathological findings are consistent with the hypothesis that in utero torsion may be an underlying factor in the pathogenesis of TRS.

Histopathologic examination has confirmed the presence of germ cells in 11% of testicular remnants. Viable germ cells or seminiferous tubules have been reported in 0 to 16% of excised testicular remnants (7,13,33). Although the precise fate of these remnants is uncertain, the presence of germ cells at least indicates a potential for germ cell–derived neoplasia. In addition, intratubular germ-cell neoplasia in a testicular remnant has been reported in at least one case (6).


Le Conte first recognized the tumor potential of the undescended testis in 1851 (34). Since then, the increased risk of malignant degeneration of the cryptorchid testis has been estimated to be 20 to 46 times greater than that of the general population (35). However, the location of these testes confers a significantly different degree of risk. Intra-abdominal testes are six times more likely to develop a testis tumor than the undescended testis located in an inguinal position (36). The infrequent finding of seminiferous tubules or other germinal tissue in the surgical specimens of patients with inguinal vanishing testes also supports the contention that the risk of malignant degeneration is quite low in this population. Cendron et al (10) recently presented a histologic evaluation of 25 vanishing testis specimens and noted no identifiable testicular elements in any of these cases.

There is a high risk of malignant transformation in intraabdominal testes especially when associated with external genitalia anomaly and chromosomal anomaly. A testis tumor may develop even after a successful orchiopexy for undescended testis. However, to date, it is not clarified whether vanishing testis and true undescended testis have same etiopathogenesis and risks. The concern in this situation raises the question about the malignant degeneration of residual germinal tissue in the testicular remnants.

Theoretically, presence of testicular tissue in testicular remnants indicates a potential for malignancy in the long term. Histopathological examination of testicular remnants in nonpalpable testes demonstrated presence of seminiferous tubules and viable germ cells in 0 to 16% of the reported series (4,6,10,36). Based on these observations, some authors suggest routine removal of testicular remnant tissue to prevent malignant transformation, but others did not accept this indication because they could not find testicular tissue in testicular remnants.


Nonpalpable testes are a heterogeneous group; their assessment for differential diagnosis needs to include subsets of absent testes, intra-abdominal testes, and testes or testicular remnants located in the inguinal canal that were missed on physical examination. Because of this heterogeneity, laparoscopy has emerged as a useful tool in the localization and assessment of the nonpalpable testis. Various series report success rates of 90% to 95% in the localization of nonpalpable testes with laparoscopy (37,38,39).

While diagnostic laparoscopy is a safe and effective method of investigating patients with a nonpalpable testis, a simpler, less invasive and less costly initial step is to first explore the scrotum. Failure to detect the gonad in the scrotum in a patient with spermatic cord duct structures may occur in two situations: the testis has regressed or the surgeon has not yet localized it. The pathologist may play a critical role in the management of these patients. 95% of the testes are localized at or below the internal inguinal ring, therefore, pathologic evaluation of the spermatic cord and confirmation of the testis as regressed in tissues removed at primary inguinal exploration can reassure the surgeon of the correct diagnosis. In the majority of the cases, this pathologic evaluation can also eliminate the necessity for further surgical intervention or for radiologic evaluation.

Some authors argue that routine exploration of the groin is required only if laparoscopy reveals an open internal ring with normal vessels, while this exploration is unnecessary if the findings show hypoplastic vessels entering a closed ring (19). Others similarly note that no further action is required if blind-ending vessels are found in the abdomen (6).


There is controversy regarding the optimal management of the testicular remnant in cases of TRS. Some urologists recommend surgical exploration, either laparoscopically or via inguinal/scrotal approach, whereas others believe that these procedures are unnecessary (10,33,39). The variable reports of viable germ cell elements found within the testicular remnants may be the reason for the differences in the opinion concerning the proper management. Previous studies have reported an incidence of 0% to 16% of viable germ cell elements within the remnants (4,6,10,36). Some authors recommend surgical fixation of the contralateral testis to prevent torsion in the remaining testicular tissue (18). Inguinal exploration could be postponed until the implantation of testicular prosthesis which is a cosmetic option for patients with unilateral or bilateral loss of testes. Thus, testicular prosthesis implantation can be performed as a first inguinal operation and testicular remnants can be removed during the same session (36).


The histological evaluation of inguinal testicular remnants suggests that these testes became ischemic and atrophied during descent in utero or shortly after birth. The optimal management of the testicular remnants is controversial. Some authors recommend routine excision of remnants, whereas others believe that this is superfluous. A review of the current literature has not identified any report of testicular germ cell tumors which appear to arise from TRS.

1. Hegarty PK,Mushtaq I,Sebire NJ. Natural history of testicular regression syndrome and consequences for clinical managementJ Pediatr UrolYear: 2006320620818947736
2. Diamond DA,Caldamone AA. The value of laparoscopy for a 106 impalpable testis relative to clinical presentationJ UrolYear: 19921486326341353545
3. Selby DM. Stocker JT,Dehner LPSexual maldevelopment syndromesPediatric PathologyPhiladelphiaPa: JB Lippincott CoYear: 1992117159
4. Spires SE,Woolums CS,Pulito AR,Spires SM. Testicular regression syndrome: a clinical and pathologic study of 11 casesArch Pathol Lab MedYear: 200012469469810782149
5. Mouriquand PD. Undescended testes in children: the paediatric urologist's point of viewEur J EndocrinolYear: 20081598386
6. Rozanski TA,Wojno KJ,Bloom DA. The remnant orchiectomyJ UrolYear: 19961557127148558712
7. Storm D,Redden T,Aguiar M,Wilkerson M,Jordan G,Sumfest J. Histologic evaluation of the testicular remnant associated with the vanishing testes syndrome: is surgical management necessaryUrologyYear: 2007701204120618158048
8. Koyama T,Nonomura K,Ameda K,Kakizaki H,Matsugase Y,Shinno Y,Kanno T,Yamashita T,Murakumo M,Koyanagi T. Laparoscopic evaluation and the management of the nonpalpable testisDiagn Ther EndoscYear: 19974697418493455
9. Elder JS. Laparoscopy for impalpable testes: significance of the patent processus vaginalisJ UrolYear: 19941527767787912744
10. Cendron M,Schned AR,Ellsworth PI. Histological evaluation of the testicular nubbin in the vanishing testis syndromeJ UrolYear: 1998160116111629719300
11. Redman F. Impalpable testis: observations based on 208 consecutive operations for undescended testisJ UrolYear: 19801243793816107387
12. Smolko MJ,Kaplan GW,Brock WA. Location and fate of the nonpalpable testis in childrenJ UrolYear: 1983129120412066133965
13. Merry C,Sweeney B,Puri P. The vanishing testis: anatomical and histological findingsEur UrolYear: 19973165679032537
14. Grady RW,Mitchell ME,Carr MC. Laparoscopic and histologic evaluation of the inguinal vanishing testisUrologyYear: 1998528668699801116
15. Kirsch AJ,Escala J,Duckett JW,Smith GH,Zderic SA,Canning DA,Snyder HM. 3rd. Surgical management of the nonpalpable testis: the Children’s Hospital of Philadelphia’s experienceJ UrolYear: 1998159134013439507881
16. Lou CC,Lin JN,Tung TC,Wang KL. Anatomical findings of the vanishing testisChanggeng YT Xue Za ZhiYear: 199417121124
17. Bar-Maor JA,Groisman G,Lam M. Antenatal torsion of the testes, a cause of vanishing testis syndromePediatr Surg IntYear: 19938236238
18. Wright JE. The atrophic testicular remnantPediatr Surg IntYear: 19861229231
19. Papparella A,Zamparelli M,Cobellis G,Amici G,Saggiomo G,Parmeggiani P,Fioretti GP. Laparoscopy for nonpalpable testis: is inguinal exploration always necessary when the cord structures exit the inguinal ringPediatr Endosu Innov TechYear: 199932933
20. Smith NM,Byard RW,Bourne AJ. Testicular regression syndrome-a pathological study of 77 casesHistopathologyYear: 1991192692721916702
21. Sutcliffe JR,Wilson-Storey D,Smith NM. Ante-natal testicular torsion: only one cause of the testicular regression syndromeJ R Coll Surg EdinbYear: 199641991018632401
22. Huff DS,Wu HY,Snyder HM 3rd,Hadziselimovic F,Blythe B,Duckett JW. Evidence in favor of the mechancal (intrauterin torsion) theory over the endocrinopathy (cryptorchidism) theory in the pathogenesis of testicular agenesisJ UrolYear: 19911466306311677689
23. Calogero AE,Garofalo MR,Barone N,De Palma A,Vicari E,Romeo R,Tumino S,D'Agata R. Spontaneous regression over time of the germinal epithelium in a Y chromosome-microdeleted patient: case reportHum ReprodYear: 2001161845184811527886
24. Rattanachaiyanont M,Phophong P,Techatraisak K,Charoenpanich P,Jitpraphai P. Embryonic testicular regression syndrome: a case reportMed Assoc ThaiYear: 199982506510
25. Grouchy J de,Gompel A,Salomon-Bernard Y,Kuttenn F,Yaneva H,Paniel JB,Le Merrer M,Roubin M,Turleau C. Embryonic testicular regression syndrome and severe mental retardation in sibsAnn GenetYear: 1985281541603879148
26. Marcantonio SM,Fechner PY,Migeon CJ,Perlman EJ,Berkovitz GD. Embryonic testicular regression sequence: a part of the clinical spectrum of 46,XY gonadal dysgenesisAm J Med GenetYear: 199449158172233
27. Josso N,Briard ML. Embryonic testicular regression syndrome: variable phenotypic expression in siblingsJ PediatrYear: 1980972002047400886
28. Coulam CB. Testicular regression syndromeObstet GynecolYear: 1979534449569795
29. Naffah J. Familial testicular regression syndromeBull Acad Natl MedYear: 19891737097142598069
30. Berkovitz GD. Clinical and pathologic spectrum of 46,XY gonadal dysgenesis: its relevance to the understanding of sex differentiationMedicine (Baltimore)Year: 1991703753831956279
31. Philibert P,Zenaty D,Audran F,Leger J,Achermann JC,Sultan C. Mutational analysis of steroidogenic factor 1 (NR5a1) in 24 boys with bilateral anorchia: a French collaborative studyHum ReprodYear: 2007223255326117940071
32. Law H,Mushtaq I,Wingrove K,Malone M,Sebire NJ. Histopathological features of testicular regression syndrome: relation to patient age and implications for managementFetal Pediatr PatholYear: 20062511912916908461
33. Van Savage JG. Avoidance of inguinal incision in laparoscopically confirmed vanishing testis syndromeJ UrolYear: 20011661421142411547102
34. Johnson DC,Woodhead DM,Pohl DR. Cryptorchidism and testicular tumorigenesisSurgeryYear: 1968639199224384890
35. Campbell HE. Incidence of malignant growth of the undescended testicleArch SurgYear: 194244353357
36. Emir H,Ayik B,Elicevik M,Buyukunal C,Danismend N,Dervisoglu S,Soylet Y. Histological evaluation of the testicular nubbins in patients with nonpalpable testis: assessment of etiology and surgical approachPediatr Surg IntYear: 200723414417031713
37. Moore RG,Peters CA,Bauer SB,Mandell J,Retik AB. Laparoscopic evaluation of the nonpalpable testis: a prospective assessment of accuracyJ UrolYear: 19941517287317905933
38. Castilho LN. Laparoscopy for the nonpalpable testis: how to interpret the endoscopic findingsJ UrolYear: 1990144121512181977929
39. Plotzker ED,Rushton HG,Belman AB,Skoog SJ. Laparoscopy for nonpalpable testes in childhood: is inguinal exploration also necessary when vas and vessels exit the inguinal ringJ UrolYear: 19921486356381353546

Article Categories:
  • Review

Keywords: Vanishing testis, nonpalpable testis, Cryptorchidism, testicular regression syndrome.

Previous Document:  Short-duration oral amiodarone for prevention of atrial fibrillation post heart surgery.
Next Document:  Long-term efficacy of insulin pump therapy in children with type 1 diabetes mellitus.