Document Detail


Vanadium compounds modulate PPARγ activity primarily by increasing PPARγ protein levels in mouse insulinoma NIT-1 cells.
MedLine Citation:
PMID:  23456093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vanadium compounds are promising agents in the therapeutic treatment of diabetes; however, their mechanism of action has not been clearly elucidated. The current study investigated the effects of vanadium compounds, vanadyl acetylacetonate [V(IV)O(acac)2] and sodium metavanadate (NaV(V)O3), on peroxisome proliferator-activated receptors (PPARs), especially PPARγ, which are important targets of anti-diabetic drugs. Our experimental results revealed that treatment of NIT-1 β-pancreas cells with vanadium compounds resulted in PPARγ activation and elevation of PPARγ protein levels. Vanadium compounds did not increase PPARγ transcription but ameliorated PPARγ degradation induced by inflammatory stimulators TNF-α/IL-6. Vanadium compounds induced binding of PPARγ to heat shock protein (Hsp60). This PPARγ-Hsp60 interaction might cause inhibition of PPARγ degradation, thus elevating the PPARγ level. In addition, modulation of PPARγ phosphorylation was also observed upon vanadium treatment. The present work demonstrated for the first time that vanadium compounds are novel PPARγ modulators. The results may provide new insights for the mechanism of anti-diabetic action of vanadium compounds.
Authors:
Pan Zhao; Xiaoda Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Metallomics : integrated biometal science     Volume:  5     ISSN:  1756-591X     ISO Abbreviation:  Metallomics     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-26     Completed Date:  2014-01-27     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101478346     Medline TA:  Metallomics     Country:  England    
Other Details:
Languages:  eng     Pagination:  836-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Chaperonin 60 / metabolism
DNA Primers
Insulinoma / metabolism*,  pathology
Mice
PPAR gamma / drug effects*,  metabolism
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Vanadium Compounds / pharmacology*
Chemical
Reg. No./Substance:
0/Chaperonin 60; 0/DNA Primers; 0/PPAR gamma; 0/Vanadium Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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