Document Detail


The Vam6 and Gtr1-Gtr2 pathway activates TORC1 in response to amino acids in fission yeast.
MedLine Citation:
PMID:  22344254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Rag family of GTPases has been implicated in the TORC1 activation in Drosophila and in mammalian cells in response to amino acids. We have investigated the role of the Rag GTPases Gtr1 and Gtr2 in TORC1 regulation in Schizosaccharomyces pombe. Fission yeast Gtr1 and Gtr2 are non-essential proteins that enhance cell growth in the presence of amino acids in the medium. The function of Gtr1 and Gtr2 in nutrient signaling is further supported by the observation that even in rich medium the deletion of either gene results in the promotion of mating, meiosis and sporulation, consistent with the downregulation of TORC1. We show that Gtr1 and Gtr2 colocalize with TORC1 in vacuoles, where TORC1 is presumably activated. Epistasis analyses indicated that Gtr1 and Gtr2 function downstream of Vam6 and upstream of TORC1 in response to amino acid signals. Our data demonstrate the existence of an evolutionarily conserved pathway with the Vam6 and Gtr1-Gtr2 pathway activating TORC1, which in turns stimulates cell growth and inhibits sexual differentiation.
Authors:
Noelia Valbuena; Kun-Liang Guan; Sergio Moreno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-17
Journal Detail:
Title:  Journal of cell science     Volume:  125     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-05-28     Completed Date:  2013-01-24     Revised Date:  2013-07-18    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1920-8     Citation Subset:  IM    
Affiliation:
Instituto de Biología Molecular y Celular del Cáncer, CSIC/University of Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Gene Expression Regulation, Fungal
Schizosaccharomyces / genetics,  metabolism*
Schizosaccharomyces pombe Proteins / genetics,  metabolism*
Signal Transduction
Vacuoles / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
R01 CA108941/CA/NCI NIH HHS; R01 GM051586/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Schizosaccharomyces pombe Proteins
Comments/Corrections

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