Document Detail


Value of the O-nitrophenyl-beta-D-galactopyranoside test to differentiate among the aerobic actinomycetes.
MedLine Citation:
PMID:  2121796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comparative study to determine beta-D-galactosidase activity among 171 strains of aerobic actinomycetes (including mycobacteria and rhodococci) was performed by using two growth media and four O-nitrophenyl-beta-D-galactopyranoside (ONPG) substrates. The ONPG test was found to be a valuable screening test to differentiate between the ONPG-positive Nocardia spp. and the rapidly growing ONPG-negative mycobacteria and rhodococci. However, ONPG results varied significantly depending on the growth medium and test substrate used.
Authors:
M Flores; E G Ford; J M Janda
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  28     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-12-13     Completed Date:  1990-12-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2142-4     Citation Subset:  IM    
Affiliation:
Microbial Diseases Laboratory, California Department of Health Services, Berkeley 94704.
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MeSH Terms
Descriptor/Qualifier:
Actinomycetales / classification,  isolation & purification,  metabolism*
Aerobiosis
Bacteriological Techniques
Culture Media
Evaluation Studies as Topic
Humans
Nitrophenylgalactosides / metabolism*
Species Specificity
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/Culture Media; 19710-96-4/2-nitrophenylgalactoside; 28347-45-7/Nitrophenylgalactosides; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

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