Document Detail

Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos.
MedLine Citation:
PMID:  20132014     Owner:  NLM     Status:  MEDLINE    
The present study was carried out to examine the effects of valproic acid (VPA), a histone deacetylase inhibitor, on in vitro development of miniature pig somatic cell nuclear transfer (SCNT) embryos and on expression of a mouse Oct-3/4 promoter-driven enhanced green fluorescent protein (EGFP) gene (EGFP expression only detected in Oct-3/4-expressing cells) introduced into donor cells for SCNT during their development. The addition of 4 mM VPA to embryo culture medium for 48 h after activation significantly (p < 0.01) increased the blastocyst formation rate of SCNT embryos compared with the control, whereas VPA did not affect their cleavage rate. The rate of SCNT embryos expressing EGFP at 5 days of culture was not affected by the presence or absence of VPA treatment. At 7 days of culture, however, the addition of 4 mM VPA to embryo culture medium for 48 h after activation significantly (p < 0.05) increased the rate of SCNT embryos expressing EGFP compared with the control. The results indicate that VPA enhances the ability of miniature pig SCNT embryos to develop into blastocysts and maintains the ability of them to express Oct-3/4 gene.
Kazuchika Miyoshi; Hironori Mori; Yamato Mizobe; Eri Akasaka; Akio Ozawa; Mitsutoshi Yoshida; Masahiro Sato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular reprogramming     Volume:  12     ISSN:  2152-4998     ISO Abbreviation:  Cell Reprogram     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-05     Completed Date:  2010-05-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101528176     Medline TA:  Cell Reprogram     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-74     Citation Subset:  IM    
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima, Japan.
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MeSH Terms
Cell Nucleus / genetics
Dose-Response Relationship, Drug
Embryo Culture Techniques / methods
Embryo Transfer / methods
Embryo, Mammalian / cytology,  metabolism
Embryonic Development / drug effects*
Enzyme Inhibitors / pharmacology*
Fluorescent Dyes / metabolism
Green Fluorescent Proteins / genetics,  metabolism
Nuclear Transfer Techniques / veterinary*
Octamer Transcription Factor-3 / genetics,  metabolism*
Swine, Miniature / embryology*
Time Factors
Transfection / veterinary
Valproic Acid / pharmacology*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Fluorescent Dyes; 0/Octamer Transcription Factor-3; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 99-66-1/Valproic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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