Document Detail


Validity of the microdialysis technique for experimental in vivo studies of myocardial energy metabolism.
MedLine Citation:
PMID:  12940939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The validity of the microdialysis technique for experimental in vivo studies of myocardial energy metabolism is not known. To address this question interstitial levels of energy-related metabolites (lactate, adenosine, inosine and hypoxanthine) obtained by the microdialysis technique were compared with corresponding metabolites from myocardial biopsies at given intervals in a porcine heart model using different protocols of ischaemia and reperfusion. METHODS: In an open chest porcine heart model, interstitial levels of energy-related metabolites were monitored using the microdialysis technique. All animals (n = 23) were subjected to 120-min pretreatment followed by 40 min of regional ischaemia and 120 min of reperfusion. Tissue biopsies were obtained in the beginning, middle and at the end of the 40-min ischaemic period and at the end of the reperfusion period. Pretreatment consisted of either rest (group 1, n = 7), or rest for 90 min and one ischaemia/reperfusion (10 + 20 min) cycle (group 2, n = 9), or four ischaemia/reperfusion cycles (10 + 20 min each) (group 3, n = 7). RESULTS: Interstitial levels of energy-related metabolites monitored by the microdialysis technique correlated with tissue biopsy levels of lactate (r = 0.90, P < 0.001), adenosine (r = 0.89, P < 0.001), inosine (r = 0.88, P < 0.001) and hypoxanthine (r = 0.91, P < 0.001), respectively, which were obtained by tissue biopsies at given time intervals. These significant correlations were valid regardless of the functional state of the myocardium. CONCLUSION: We observed significant correlations between microdialysis probe levels and tissue biopsy levels of energy-related metabolites in both ischaemic and non-ischaemic tissue. These data assess the validity of the microdialysis technique (in the current setting) for studying dynamic changes of myocardial energy metabolism.
Authors:
M Kavianipour; G Wikström; G Ronquist; A Waldenström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Acta physiologica Scandinavica     Volume:  179     ISSN:  0001-6772     ISO Abbreviation:  Acta Physiol. Scand.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-27     Completed Date:  2003-12-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370362     Medline TA:  Acta Physiol Scand     Country:  England    
Other Details:
Languages:  eng     Pagination:  61-5     Citation Subset:  IM    
Affiliation:
Department of Cardiology, Norrland University Hospital, Umeå, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenosine / metabolism
Animals
Biopsy
Energy Metabolism*
Hypoxanthine / metabolism
Inosine / metabolism
Lactic Acid / metabolism
Microdialysis / methods*
Myocardial Ischemia / metabolism
Myocardial Reperfusion
Myocardium / metabolism*
Reproducibility of Results
Swine
Chemical
Reg. No./Substance:
50-21-5/Lactic Acid; 58-61-7/Adenosine; 58-63-9/Inosine; 68-94-0/Hypoxanthine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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