| Validity of triple- and dual-tracer techniques to estimate glucose appearance. | |
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MedLine Citation:
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PMID: 22454288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The triple-tracer (TT) dilution technique has been proposed to be the gold standard method to measure postprandial glucose appearance. However, validation against an independent standard has been missing. We addressed this issue and also validated the simpler dual-tracer (DT) technique. Sixteen young subjects with type 1 diabetes (age 19.5 ± 3.8 yr, BMI 23.4 ± 1.5 kg/m(2), HbA(1c) 8.7 ± 1.7%, diabetes duration 9.0 ± 6.9 yr, total daily insulin 0.9 ± 0.2 U·kg(-1)·day(-1), mean ± SD) received a variable intravenous 20% dextrose infusion enriched with [U-(13)C]glucose over 8 h to achieve postprandial-resembling glucose excursions while intravenous insulin was administered to achieve postprandial-resembling levels of plasma insulin. Primed [6,6-(2)H(2)]glucose was infused in a manner that mimicked the expected endogenous glucose production and [U-(13)C; 1,2,3,4,5,6,6-(2)H(7)]glucose was infused in a manner that mimicked the expected glucose appearance from a standard meal. Plasma glucose enrichment was measured by gas chromatography-mass spectrometry. The intravenous dextrose infusion served as an independent standard and was reconstructed using the TT and DT techniques with the two-compartment Radziuk/Mari model and an advanced stochastic computational method. The difference between the infused and reconstructed dextrose profile was similar for the two methods (root mean square error 6.6 ± 1.9 vs. 8.0 ± 3.5 μmol·kg(-1)·min(-1), TT vs. DT, P = NS, paired t-test). The TT technique was more accurate in recovering the overall dextrose infusion (100 ± 9 and 92 ± 12%; P = 0.02). The root mean square error associated with the mean dextrose infusion profile was 2.5 and 3.3 μmol·kg(-1)·min(-1) for the TT and DT techniques, respectively. We conclude that the TT and DT techniques combined with the advanced computational method can measure accurately exogenous glucose appearance. The TT technique tends to outperform slightly the DT technique, but the latter benefits from reduced experimental and computational complexity. |
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Authors:
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A Haidar; D Elleri; J M Allen; J Harris; K Kumareswaran; M Nodale; C L Acerini; M E Wilinska; N Jackson; A M Umpleby; M L Evans; D B Dunger; R Hovorka |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-03-27 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: 302 ISSN: 1522-1555 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-06-18 Completed Date: 2012-08-27 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: E1493-501 Citation Subset: IM |
Affiliation:
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Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Algorithms Area Under Curve Blood Glucose / metabolism Carbon Radioisotopes / chemistry, diagnostic use Data Interpretation, Statistical Deuterium / chemistry, diagnostic use Female Gas Chromatography-Mass Spectrometry Glucose / metabolism*, pharmacology Hemoglobin A, Glycosylated / analysis Humans Infusions, Intravenous Insulin / blood Intestinal Absorption Isotope Labeling Least-Squares Analysis Male Radioactive Tracers* Radioisotope Dilution Technique* Reproducibility of Results Stochastic Processes Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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1R01 DK-085621/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Carbon Radioisotopes; 0/Hemoglobin A, Glycosylated; 0/Insulin; 0/Radioactive Tracers; 50-99-7/Glucose; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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