| Validation of a toxicity testing model by evaluating oxygen supply and energy state in the isolated perfused rat kidney. Single-pass preparation without albumin. | |
| | |
MedLine Citation:
|
PMID: 2056752 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Validation of a toxicity testing model concerning energy metabolism was attempted by evaluating the oxygen supply and energy state in an isolated perfused rat kidney of single-pass preparation without albumin. Perfusion was performed at a temperature of 31 degrees C, flow rate of 11.0 mL/g/min, and pressure of 81-104 mmHg. The perfusate was saturated with 95% O2/5% CO2. After preperfusion for 30 min, the redox states of cytochrome aa3 and c and pyridine nucleotides (PN) in the perfused kidney were measured to be stable for 90 min by a scanning reflectance spectrophotometry and surface fluorometry, respectively. During the same period, the contents of ATP and inorganic phosphate (Pi) in the perfused kidney were also measured to be stable by 31P-NMR spectroscopy. The oxygen supply to the cell was more than the amount required for the basal metabolism of the cell. For assessment of the effects of chemical agents on the renal cell metabolism, this preparation of the perfused rat kidney was considered to have several advantages, despite some of its inherent limitations in the function of the kidney. |
| | |
Authors:
|
T Takano; K Nakata; T Kawakami; Y Miyazaki; M Murakami; Y Seo; E Suzuki |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of pharmacological methods Volume: 25 ISSN: 0160-5402 ISO Abbreviation: J Pharmacol Methods Publication Date: 1991 May |
Date Detail:
|
Created Date: 1991-07-30 Completed Date: 1991-07-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7806596 Medline TA: J Pharmacol Methods Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 195-204 Citation Subset: IM |
Affiliation:
|
Department of Public Health and Environmental Science, School of Medicine, Tokyo Medical and Dental University, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
metabolism Albumins / pharmacology Animals Energy Metabolism* Female Kidney / drug effects, metabolism* Magnetic Resonance Spectroscopy Oxygen Consumption* Perfusion Rats Rats, Inbred Strains Toxicology / methods* |
| Chemical | |
Reg. No./Substance:
|
0/Albumins; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Screening for potassium channel modulators by a high through-put 86-rubidium efflux assay in a 96-we...
Next Document: The increasing-temperature hot-plate test: an improved test of nociception in mice and rats.