Document Detail

Validation of swabs as a non-destructive and relatively non-invasive DNA sampling method in fish.
MedLine Citation:
PMID:  21429106     Owner:  NLM     Status:  In-Data-Review    
Non-destructive methods of collecting DNA from small fish species can be problematic, as fin clips can potentially affect behaviour or survivorship in the wild. Swabbing body mucus may provide a less invasive method of DNA collection. However, risk of contamination from other individuals in high density groups could give erroneous genotyping results. We compared multilocus microsatellite genotypes from the same individuals when collected at low and high density and compared this with fin clips. We found no differences between these categories, with a genotyping error rate of 0.42%, validating the use of body mucus swabbing for DNA collection in fish.
A L LE Vin; A Adam; A Tedder; K E Arnold; B K Mable
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Publication Detail:
Type:  Journal Article     Date:  2010-08-18
Journal Detail:
Title:  Molecular ecology resources     Volume:  11     ISSN:  1755-0998     ISO Abbreviation:  Mol Ecol Resour     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-03-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465604     Medline TA:  Mol Ecol Resour     Country:  England    
Other Details:
Languages:  eng     Pagination:  107-9     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Division of Ecology and Evolutionary Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK Environment Department, University of York, York, YO10 5DD, UK.
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