Document Detail


Validation of a serum immunoassay to measure progesterone and diagnose pregnancy in the West Indian manatee (Trichechus manatus).
MedLine Citation:
PMID:  18760463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective was to validate a high-sensitivity chemiluminescent assay of serum progesterone concentrations for pregnancy diagnosis in manatees. Assay analytical sensitivity was 0.1 ng/mL, with mean intra- and inter-assay coefficients of variation of 9.7 and 9.2%, respectively, and accuracy had a mean adjusted R(2) of 0.98. Methods comparison (relative to Siemen's Coat-A-Count RIA) demonstrated r=0.98, Deming regression slope of 0.95, and an intercept of 0.01. Based on ROC analysis, a progesterone concentration >or=0.4 ng/mL was indicative of pregnancy. Assay results were not significantly altered by two freeze-thaw cycles of samples. Characteristic progesterone concentrations during pregnancy were Months 1-4 (1.7-4.7 ng/mL), 5-8 ( approximately 1.0 ng/mL), and 10 and 11 (0.3-0.5 ng/mL), whereas two late-pregnant females with impending abortion had progesterone concentrations of 0.1 ng/mL. Among pregnant females, maximum progesterone concentrations occurred in autumn (3.9+/-1.8 ng/mL), and were greater during all seasons than concentrations in non-pregnant females (0.1-0.2 ng/mL). Progesterone concentrations were also significantly higher in pregnant females than in non-pregnant females and males. This highly sensitive, specific, and diagnostic assay will be valuable for monitoring pregnancy and abortion in manatees.
Authors:
K M Tripp; J P Verstegen; C J Deutsch; R K Bonde; M Rodriguez; B Morales; D L Schmitt; K E Harr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-08-29
Journal Detail:
Title:  Theriogenology     Volume:  70     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1030-40     Citation Subset:  IM    
Affiliation:
University of Florida College of Veterinary Medicine, Aquatic Animal Health Program, 2015 SW 16th Avenue, P.O. Box 100126, Gainesville, FL 32610-0126, USA. ktripp@savethemanatee.org
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MeSH Terms
Descriptor/Qualifier:
Abortion, Veterinary / blood
Age Distribution
Animals
Female
Immunoassay / methods,  veterinary*
Male
Pregnancy
Pregnancy, Animal / blood
Progesterone / blood*
Reproducibility of Results
Sensitivity and Specificity
Sex Characteristics
Specimen Handling
Trichechus manatus / blood*
Chemical
Reg. No./Substance:
57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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