Document Detail


Validation of octanoate breath test for measuring gastric emptying in rats.
MedLine Citation:
PMID:  23667748     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Lack of simple and repeatable tests hampers gastric emptying studies in rats. The aim of this study was to adapt the (14)C-octanoate solid gastric emptying breath test for application in rats, and to validate it against radioscintigraphic method.
METHODS: After ingestion of a meal containing 3 mCi (99m)Tc and 2 µCi (14)C-octanoate, 23 male Wistar rats were placed on a gamma camera in a airflow container. Scintigraphic images were taken at regular intervals. The amount of (14)CO2 in a regularly replaced hyamine hydroxide solution, capturing CO2 in the outflow air, was counted using liquid scintillation spectrometry. (99m)Tc gastric retention curves and (14)CO2-excretion curves were fitted to their respective data. Three rats underwent the same procedures after administration of atropine.
RESULTS: Overall Tr10% (time at which 10% of the original amount of (99m)Tc remained in the stomach) was 355 ± 64 minutes; Te90% (time at which 90% of total amount of (14)CO2 was excreted) was 325 ± 106 minutes. Their correlation coefficient was 0.71, R-square 0.50 and P < 0.005. Tr1/2 (50% of original amount of (99m)Tc remained) was 124 ± 28 minutes; Te1/2 (50% of total amount of (14)CO2 excreted) 114 ± 32 minutes. Their correlation coefficient was 0.83 with R-square of 0.69 and P < 0.00005. In 12 immobilized animals correlation was even better: correlation coefficient 0.84; R-square 0.71 and P < 0.001 (Tr10% was 388 ± 117 minutes; Te90% 532 ± 219 minutes; Tr1/2 of 165 ± 54 minutes; Te1/2 of 175 ± 67 minutes). Atropine significantly lengthened all emptying times: 904 ± 307 and 1461 ± 684 minutes for Tr10% and Te90%, respectively; and 432 ± 117 minutes for Tr1/2 and 473 ± 190 minutes for Te1/2.
CONCLUSIONS: We adapted and validated the (14)C-octanoate gastric emptying breath test for application in rats.
Authors:
Ingrid Demedts; Christophe Vanormelingen; Hubert Van Billoen; Tim Vanuytsel; Ricard Farré; Tatsuhiro Masaoka; Alfons Verbruggen; Kristien Verbeke; Pieter Vanden Berghe; Jan Tack
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Publication Detail:
Type:  Journal Article     Date:  2013-04-16
Journal Detail:
Title:  Journal of neurogastroenterology and motility     Volume:  19     ISSN:  2093-0879     ISO Abbreviation:  J Neurogastroenterol Motil     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-05-13     Completed Date:  2013-05-14     Revised Date:  2013-11-07    
Medline Journal Info:
Nlm Unique ID:  101530189     Medline TA:  J Neurogastroenterol Motil     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  171-8     Citation Subset:  -    
Affiliation:
Translational Research Center for Gastrointestinal Disorders, University of Leuven, Leuven, Belgium.
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