Document Detail


Validation of a novel epicutaneous delivery system for patch testing of house dust mite-hypersensitive dogs.
MedLine Citation:
PMID:  23050930     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background - Patch tests with allergens are used for the evaluation of cellular hypersensitivity to food and environmental allergens in dogs and humans with atopic dermatitis. Viaskin is a novel allergen epicutaneous delivery system that enhances epidermal allergen capture by immune cells. Objectives - To compare the use of Viaskin and Finn chamber patch tests in dogs hypersensitive to mite allergens. Methods - Empty control or Dermatophagoides farinae house dust mite-containing Viaskin or Finn chamber patches were applied to the thoracic skin of six mite-hypersensitive Maltese-beagle crossbred atopic dogs. Lesions were graded 49 and 72 h after patch test application, and skin biopsies were collected after 72 h. Overall microscopic inflammation, eosinophil and T-lymphocyte infiltrations were scored. Results - Positive macroscopic patch test reactions developed at five of six Viaskin application sites and four of six Finn chamber application sites. Median microscopic epidermal and dermal inflammation, as well as eosinophil and CD3 T-lymphocyte dermal scores were always higher in biopsies collected at Viaskin than at Finn chamber sites. Microscopic inflammation scores were significantly higher after mite allergen-containing Viaskin compared with empty patches, but this was not the case for mite-containing Finn chambers compared with control chambers. Scores obtained using Viaskin were not significantly different from those obtained using Finn chambers. Macroscopic and microscopic scores were significantly correlated. Conclusions and clinical importance - In mite-allergic dogs, Viaskin epicutaneous delivery systems appear to induce stronger allergen-specific inflammation than currently used Finn chamber patch tests. Consequently, Viaskin patches might offer a better alternative for screening cellular hypersensitivity to food and environmental allergens.
Authors:
Thierry Olivry; Keith E Linder; Judy S Paps; Petra Bizikova; Stan Dunston; Nathalie Donne; Lucie Mondoulet
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-11
Journal Detail:
Title:  Veterinary dermatology     Volume:  -     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. Veterinary Dermatology © 2012 ESVD and ACVD, Veterinary Dermatology.
Affiliation:
Department of Clinical Sciences, College of Veterinary Medicine Center for Comparative Medicine and Translational Research Department of Population Health and Pathobiology, North Carolina State University, Raleigh, NC, USA DBV Technologies, 80/84 rue des Meuniers, 92220 Bagneux, France.
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