Document Detail


Validation of a new pregnancy-associated glycoprotein radioimmunoassay method for the detection of early pregnancy in ewes.
MedLine Citation:
PMID:  19726076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the current study was to describe the use of a pool of different antisera raised against pregnancy-associated glycoproteins (PAGs; purified from both ovine and caprine placentas) for early pregnancy diagnosis in ovine species. Sixty-three pluriparous Sarda ewes (Ovis aries) were synchronized. Blood samples were withdrawn on Days 18, 24, 26, 28, 30, and 50 after mating. These samples were assayed for progesterone (radioimmunoassay [RIA] including an extraction step) and for pregnancy-associated glycoproteins (RIA-706 and RIA-srPool). Progesterone concentrations were under 1.0 ng/mL in all nonpregnant Sarda ewes. In pregnant ewes, mean progesterone concentrations ranged from 2.4 ng/mL (Day 24, single pregnancies) to 4.4 ng/mL (Day 28, multiple pregnancies). During all periods of examination, PAGs remained lower than 0.8 ng/mL in nonpregnant ewes. On Day 18 of pregnancy, PAG concentrations could be detected in 26 of 43 (60.5%) and in 41 of 43 (95.3%) pregnant ewes using the RIA-706 and RIA-srPool methods, respectively. From Day 24 to Day 50, using both RIA methods, PAGs could be detected in all pregnant ewes. On Day 24, the best threshold for pregnancy diagnosis was obtained by use of RIA-srPool, maximal concentration in nonpregnant ewes being 0.3 ng/mL and minimal concentration in pregnant ewes being 4.8 ng/mL. In general, progesterone and PAG concentrations were higher in multiple pregnancies than in single pregnancies. However, because of large individual variations, single pregnancies could not be differentiated from multiple pregnancies.
Authors:
O Barbato; N M Sousa; A Debenedetti; C Canali; L Todini; J F Beckers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-01
Journal Detail:
Title:  Theriogenology     Volume:  72     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-21     Completed Date:  2009-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  993-1000     Citation Subset:  IM    
Affiliation:
Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy. barbato@unipg.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Glycoproteins / blood*
Pregnancy
Pregnancy Proteins / blood*
Pregnancy Tests / methods,  veterinary*
Progesterone / blood
Radioimmunoassay / methods,  veterinary*
Sensitivity and Specificity
Sheep*
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Pregnancy Proteins; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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