Document Detail

Validation of a monoclonal antibody-based ELISA to detect antibodies directed against swine vesicular disease virus.
MedLine Citation:
PMID:  9820579     Owner:  NLM     Status:  MEDLINE    
A simple, rapid and sensitive competitive monoclonal antibody-based ELISA for the detection of antibodies directed against swine vesicular disease virus (SVDV) was developed. The ELISA was validated using field sera originating from SVDV-infected and non-infected Dutch pig herds, reference sera obtained from the Community Reference Laboratory for Swine Vesicular Disease at the Institute for Animal Health, Pirbright Laboratory, UK, and sera from animals infected experimentally. When testing 4277 sera originating from non-infected Dutch pig herds and collected as part of the national screening program, this ELISA had only 0.6% false positive results, whereas approximately 2% of false positive results were obtained with a conventional blocking ELISA used until recently. A sensitivity relative to the virus neutralisation test of > 97% was achieved when testing sera collected from Dutch pig farms where an outbreak of SVDV had occurred. All international reference sera scored consistently correct. Sera collected sequentially from pigs experimentally infected with SVDV isolates representing all currently recognized antigenic groups, were scored positive slightly earlier by the ELISA compared to the virus neutralisation test. This monoclonal antibody-based competitive ELISA for SVDV antibodies designated the Ceditest ELISA for SVDV-Ab, is as sensitive but more specific than the ELISA used until recently. Because sera are tested at a single dilution (1:5), incubations are carried out at room temperature and test results are available within 3 h, this ELISA is simple, easy to automate and therefore very suitable for screening large numbers of serum samples.
G Chénard; M Bloemraad; J A Kramps; C Terpstra; A Dekker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virological methods     Volume:  75     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  105-12     Citation Subset:  IM    
Institute for Animal Science and Health, Lelystad, Netherlands.
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MeSH Terms
Antibodies, Monoclonal / immunology*
Antibodies, Viral / immunology*
Antigens, Viral / blood*
Enterovirus / immunology*
Enzyme-Linked Immunosorbent Assay / methods*,  standards
Sensitivity and Specificity
Swine Vesicular Disease / blood,  immunology,  virology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Antigens, Viral

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