Document Detail


Validation of liquid chromatographic method for assay of calcitriol and alfacalcidol in capsule formulations.
MedLine Citation:
PMID:  3804943     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The validation of a liquid chromatographic procedure suitable for the determination of calcitriol and alfacalcidol in their respective formulations labeled to contain at least 0.25 micrograms drug per unit is described. The capsule content is diluted and chromatographed in 15-20 min on silica columns (5 micron) with a mobile phase of hexane-tetrahydrofuran-methylene dichloride-isopropanol (72 + 12 + 12 + 4, v/v) with detection at 254 nm. The calibration curve is linear. Recoveries of "spikes" averaged 101% with a standard deviation of 2%. Precision was better than 1.5%.
Authors:
B C Flann; B A Lodge
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  69     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1986 Nov-Dec
Date Detail:
Created Date:  1987-03-04     Completed Date:  1987-03-04     Revised Date:  2013-05-27    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1026-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Calcitriol / analysis*
Capsules
Chromatography, Liquid
Hydroxycholecalciferols / analysis*
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Capsules; 0/Hydroxycholecalciferols; 32222-06-3/Calcitriol; URQ2517572/alfacalcidol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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