| Validation of liquid chromatographic method for assay of calcitriol and alfacalcidol in capsule formulations. | |
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MedLine Citation:
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PMID: 3804943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The validation of a liquid chromatographic procedure suitable for the determination of calcitriol and alfacalcidol in their respective formulations labeled to contain at least 0.25 micrograms drug per unit is described. The capsule content is diluted and chromatographed in 15-20 min on silica columns (5 micron) with a mobile phase of hexane-tetrahydrofuran-methylene dichloride-isopropanol (72 + 12 + 12 + 4, v/v) with detection at 254 nm. The calibration curve is linear. Recoveries of "spikes" averaged 101% with a standard deviation of 2%. Precision was better than 1.5%. |
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Authors:
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B C Flann; B A Lodge |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal - Association of Official Analytical Chemists Volume: 69 ISSN: 0004-5756 ISO Abbreviation: J Assoc Off Anal Chem Publication Date: 1986 Nov-Dec |
Date Detail:
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Created Date: 1987-03-04 Completed Date: 1987-03-04 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 7505559 Medline TA: J Assoc Off Anal Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1026-30 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcitriol
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analysis* Capsules Chromatography, Liquid Hydroxycholecalciferols / analysis* Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Capsules; 0/Hydroxycholecalciferols; 32222-06-3/Calcitriol; 41294-56-8/1-hydroxycholecalciferol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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