| Validation in an animal model of the carbon 13-labeled mixed triglyceride breath test for the detection of intestinal fat malabsorption. | |
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MedLine Citation:
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PMID: 10518078 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine, in a rat model of fat malabsorption, the potency of the carbon 13-labeled mixed triglyceride ((13)C-MTG) breath test as a noninvasive, patient-friendly replacement for classic fat balance studies. Study design: Comparison of the percentage of fat absorption, detected by fat balance, with the (13)CO(2) recovery of the (13)C-MTG breath test in rats fed high-fat chow and varying amounts of the lipase inhibitor, orlistat (0, 50, 200, and 800 mg per kilogram of chow), for 5 days. RESULTS: On orlistat administration, total fat absorption decreased from 80.2% +/- 2.2% to 32.8% +/- 3.7% (mean +/- SEM, 0 mg and 800 mg of orlistat per kilogram of chow, respectively; P <.001). Correspondingly, breath (13)CO(2) recovery from (13)C-MTG at 6 hours decreased from 84.5% +/- 7.8% to 42.0% +/- 1.5% of the dose (0 mg and 800 mg of orlistat per kilogram of chow, respectively; P <.001). The 6-hour recovery of breath (13)CO(2) appeared to be highly correlated with the percentage of fat absorption (r = 0.88, P <.001). In rats with fat absorption higher than 70%, however, the coefficient of variation of the (13)C-MTG breath test was 3-fold larger than that of the fat balance. CONCLUSIONS: The (13)C-MTG breath test could potentially replace the fat balance method for comparing fat absorption efficacy between groups. Yet, a considerable interindividual variation of the (13)C-MTG breath test under conditions of relatively mild fat malabsorption does not support its application for diagnostic purposes in individuals. |
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Authors:
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M Kalivianakis; J Elstrodt; R Havinga; F Kuipers; F Stellaard; P J Sauer; R J Vonk; H J Verkade |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pediatrics Volume: 135 ISSN: 0022-3476 ISO Abbreviation: J. Pediatr. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-11-04 Completed Date: 1999-11-04 Revised Date: 2009-11-03 |
Medline Journal Info:
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Nlm Unique ID: 0375410 Medline TA: J Pediatr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 444-50 Citation Subset: AIM; IM |
Affiliation:
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Groningen University Institute for Drug Exploration, Center for Liver, Digestive and Metabolic Diseases, Department of Pediatrics, University Hospital Groningen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breath Tests* Carbon Dioxide / analysis Carbon Isotopes* Chromatography, Gas Dietary Fats / metabolism* Enzyme Inhibitors / pharmacology Fats / analysis Feces / chemistry Gas Chromatography-Mass Spectrometry Intestinal Absorption* Lactones / pharmacology Lipase / antagonists & inhibitors Lipids / blood Malabsorption Syndromes / diagnosis*, metabolism Male Rats Rats, Wistar Triglycerides / diagnostic use* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Dietary Fats; 0/Enzyme Inhibitors; 0/Fats; 0/Lactones; 0/Lipids; 0/Triglycerides; 124-38-9/Carbon Dioxide; 96829-58-2/orlistat; EC 3.1.1.3/Lipase |
| Comments/Corrections | |
Comment In:
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J Pediatr. 1999 Oct;135(4):407-9
[PMID:
10518070
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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