Document Detail


Validation in an animal model of the carbon 13-labeled mixed triglyceride breath test for the detection of intestinal fat malabsorption.
MedLine Citation:
PMID:  10518078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To determine, in a rat model of fat malabsorption, the potency of the carbon 13-labeled mixed triglyceride ((13)C-MTG) breath test as a noninvasive, patient-friendly replacement for classic fat balance studies. Study design: Comparison of the percentage of fat absorption, detected by fat balance, with the (13)CO(2) recovery of the (13)C-MTG breath test in rats fed high-fat chow and varying amounts of the lipase inhibitor, orlistat (0, 50, 200, and 800 mg per kilogram of chow), for 5 days. RESULTS: On orlistat administration, total fat absorption decreased from 80.2% +/- 2.2% to 32.8% +/- 3.7% (mean +/- SEM, 0 mg and 800 mg of orlistat per kilogram of chow, respectively; P <.001). Correspondingly, breath (13)CO(2) recovery from (13)C-MTG at 6 hours decreased from 84.5% +/- 7.8% to 42.0% +/- 1.5% of the dose (0 mg and 800 mg of orlistat per kilogram of chow, respectively; P <.001). The 6-hour recovery of breath (13)CO(2) appeared to be highly correlated with the percentage of fat absorption (r = 0.88, P <.001). In rats with fat absorption higher than 70%, however, the coefficient of variation of the (13)C-MTG breath test was 3-fold larger than that of the fat balance. CONCLUSIONS: The (13)C-MTG breath test could potentially replace the fat balance method for comparing fat absorption efficacy between groups. Yet, a considerable interindividual variation of the (13)C-MTG breath test under conditions of relatively mild fat malabsorption does not support its application for diagnostic purposes in individuals.
Authors:
M Kalivianakis; J Elstrodt; R Havinga; F Kuipers; F Stellaard; P J Sauer; R J Vonk; H J Verkade
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pediatrics     Volume:  135     ISSN:  0022-3476     ISO Abbreviation:  J. Pediatr.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-04     Completed Date:  1999-11-04     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0375410     Medline TA:  J Pediatr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  444-50     Citation Subset:  AIM; IM    
Affiliation:
Groningen University Institute for Drug Exploration, Center for Liver, Digestive and Metabolic Diseases, Department of Pediatrics, University Hospital Groningen, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Breath Tests*
Carbon Dioxide / analysis
Carbon Isotopes*
Chromatography, Gas
Dietary Fats / metabolism*
Enzyme Inhibitors / pharmacology
Fats / analysis
Feces / chemistry
Gas Chromatography-Mass Spectrometry
Intestinal Absorption*
Lactones / pharmacology
Lipase / antagonists & inhibitors
Lipids / blood
Malabsorption Syndromes / diagnosis*,  metabolism
Male
Rats
Rats, Wistar
Triglycerides / diagnostic use*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Dietary Fats; 0/Enzyme Inhibitors; 0/Fats; 0/Lactones; 0/Lipids; 0/Triglycerides; 124-38-9/Carbon Dioxide; 96829-58-2/orlistat; EC 3.1.1.3/Lipase
Comments/Corrections
Comment In:
J Pediatr. 1999 Oct;135(4):407-9   [PMID:  10518070 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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