Document Detail


Validation of a high performance liquid chromatography-tandem mass spectrometry method for β-hydroxy fatty acids as environmental markers of lipopolysaccharide.
MedLine Citation:
PMID:  24508354     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The most commonly analytical method used for the assessment of the occupational exposure to airborne endotoxins is the LAL test, but other methods are reported in the literature. The objective of this work was to develop and validate a method using HPLC coupled to triple quadrupole mass spectrometer for the quantitative determination of β-hydroxy-dodecanoic and β-hydroxy-tetradecanoic acids as chemical markers of lipopolysaccharide in Gram-negative strains. Matrix effect was significant but compensated by the use of internal standard; accuracy was always more than 85% and variability always less than 10%. LOD and LLOQ were respectively 2.5μg/l and 10μg/l for β-hydroxy-dodecanoic acid, 1μg/l and 5μg/l for β-hydroxy-tetradecanoic acid. The analysis of different bacterial strains showed that samples contain β-hydroxy-dodecanoic and β-hydroxy-tetradecanoic acids in variable ratio, and therefore the sum of their concentrations was considered the chemical marker. The method showed a significant correlation of results with those of LAL-test on four bacterial strains.
Authors:
Alessandra Chiominto; Anna Maria Marcelloni; Giovanna Tranfo; Emilia Paba; Enrico Paci
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-1-24
Journal Detail:
Title:  Journal of chromatography. A     Volume:  -     ISSN:  1873-3778     ISO Abbreviation:  J Chromatogr A     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2014-2-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier B.V. All rights reserved.
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