| Validation of a high-performance liquid chromatography analytical method for ochratoxin A quantification in cocoa beans. | |
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MedLine Citation:
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PMID: 15764339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A validated high-performance liquid chromatography (HPLC) method with fluorescence detection for the quantitative analysis of ochratoxin A (OTA) in cocoa beans is described. OTA was extracted with methanol-3% sodium hydrogen carbonate solution and then purified with immunoaffinity columns before its analysis by HPLC. The validation of the analytical method was based on the following criteria: selectivity, linearity, limit of detection and quantification, precision (within- and between-day variability) and recovery, robustness and uncertainty. Detection and quantification limits were 0.04 and 0.1 mug kg(-1), respectively. Recovery was 88.9% (relative standard deviation = 4.0%). This method was successfully applied to the measurement of 46 cocoa bean samples of different origins. A total of 63% of cocoa bean samples was contaminated with a level greater than the limit of detection. The means and medians obtained for cocoa bean were 1.71 and 1.12 mug kg(-1), respectively. Surveillance controls should be set up in both crops and factories involved in transformation processes to avoid this mycotoxin in final products. |
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Authors:
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S Amézqueta; E González-Peñas; M Murillo; A López de Cerain |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Food additives and contaminants Volume: 21 ISSN: 0265-203X ISO Abbreviation: Food Addit Contam Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2005-03-14 Completed Date: 2005-06-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8500474 Medline TA: Food Addit Contam Country: England |
Other Details:
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Languages: eng Pagination: 1096-106 Citation Subset: IM |
Affiliation:
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Organic and Pharmaceutical Chemistry Department, University of Navarra, E-31008 Pamplona, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cacao
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chemistry* Chromatography, High Pressure Liquid / methods* Food Analysis / methods Food Contamination / analysis* Humans Mycotoxins / analysis Ochratoxins / analysis* Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/Mycotoxins; 0/Ochratoxins; 303-47-9/ochratoxin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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