| Validation of deuterium-labeled fatty acids for the measurement of dietary fat oxidation during physical activity. | |
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MedLine Citation:
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PMID: 15342677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Measurement of 13C-labeled fatty acid oxidation is hindered by the need for acetate correction, measurement of the rate of CO2 production in a controlled environment, and frequent collection of breath samples. The use of deuterium-labeled fatty acids may overcome these limitations. Herein, d31-palmitate was validated against [1-13C]palmitate during exercise. Thirteen subjects with body mass index of 22.9 +/- 3 kg/m2 and body fat of 19.6 +/- 11% were subjected to 2 or 4 h of exercise at 25% maximum volume oxygen consumption (VO2max). The d31-palmitate and [1-13C] palmitate were given orally in a liquid meal at breakfast. The d3-acetate and [1-13C]acetate were given during another visit for acetate sequestration correction. Recovery of d31-palmitate in urine at 9 h after dose was compared with [1-13C] palmitate recovery in breath. Cumulative recovery of d31-palmitate was 10.6 +/- 3% and that of [1-13C]palmitate was 5.6 +/- 2%. The d3-acetate and [1-13C]acetate recoveries were 85 +/- 4% and 54 +/- 4%, respectively. When [1-13C]acetate recovery was used to correct 13C data, the average recovery differences were 0.4 +/- 3%. Uncorrected d31-palmitate and acetate-corrected [1-13C]palmitate were well correlated (y=0.96x + 0; P <0.0001) when used to measure fatty acid oxidation during exercise. Thus, d31-palmitate can be used in outpatient settings as it eliminates the need for acetate correction and frequent sampling. |
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Authors:
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Aarthi Raman; Stephane Blanc; Alexandra Adams; Dale A Schoeller |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2004-09-01 |
Journal Detail:
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Title: Journal of lipid research Volume: 45 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-19 Completed Date: 2005-04-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 2339-44 Citation Subset: IM |
Affiliation:
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Interdepartmental Program in Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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metabolism Deuterium / metabolism* Dietary Fats / metabolism* Exercise / physiology* Fatty Acids / metabolism* Female Humans Male Oxidation-Reduction Palmitic Acid / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK-30031/DK/NIDDK NIH HHS; RR03186/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Fatty Acids; 57-10-3/Palmitic Acid; 64-19-7/Acetic Acid; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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