Document Detail

Validation of deuterium-labeled fatty acids for the measurement of dietary fat oxidation during physical activity.
MedLine Citation:
PMID:  15342677     Owner:  NLM     Status:  MEDLINE    
Measurement of 13C-labeled fatty acid oxidation is hindered by the need for acetate correction, measurement of the rate of CO2 production in a controlled environment, and frequent collection of breath samples. The use of deuterium-labeled fatty acids may overcome these limitations. Herein, d31-palmitate was validated against [1-13C]palmitate during exercise. Thirteen subjects with body mass index of 22.9 +/- 3 kg/m2 and body fat of 19.6 +/- 11% were subjected to 2 or 4 h of exercise at 25% maximum volume oxygen consumption (VO2max). The d31-palmitate and [1-13C] palmitate were given orally in a liquid meal at breakfast. The d3-acetate and [1-13C]acetate were given during another visit for acetate sequestration correction. Recovery of d31-palmitate in urine at 9 h after dose was compared with [1-13C] palmitate recovery in breath. Cumulative recovery of d31-palmitate was 10.6 +/- 3% and that of [1-13C]palmitate was 5.6 +/- 2%. The d3-acetate and [1-13C]acetate recoveries were 85 +/- 4% and 54 +/- 4%, respectively. When [1-13C]acetate recovery was used to correct 13C data, the average recovery differences were 0.4 +/- 3%. Uncorrected d31-palmitate and acetate-corrected [1-13C]palmitate were well correlated (y=0.96x + 0; P <0.0001) when used to measure fatty acid oxidation during exercise. Thus, d31-palmitate can be used in outpatient settings as it eliminates the need for acetate correction and frequent sampling.
Aarthi Raman; Stephane Blanc; Alexandra Adams; Dale A Schoeller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-09-01
Journal Detail:
Title:  Journal of lipid research     Volume:  45     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-04-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2339-44     Citation Subset:  IM    
Interdepartmental Program in Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, USA.
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MeSH Terms
Acetic Acid / metabolism
Deuterium / metabolism*
Dietary Fats / metabolism*
Exercise / physiology*
Fatty Acids / metabolism*
Palmitic Acid / metabolism
Grant Support
Reg. No./Substance:
0/Dietary Fats; 0/Fatty Acids; 57-10-3/Palmitic Acid; 64-19-7/Acetic Acid; 7782-39-0/Deuterium

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