| Validation of bromodeoxyuridine immunohistochemistry for localization of S-phase cells in decalcified tissues. A comparative study with tritiated thymidine autoradiography. | |
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MedLine Citation:
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PMID: 2228729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunohistochemical detection of the thymidine analogue 5-bromo-2'-deoxyuridine (BrdUrd), which is incorporated by S-phase cells, offers a convenient way of studying the proliferation kinetics of cells in normal skeletal tissues and in bone containing/derived tumours. To assess the validity of using this approach on decalcified, paraffin embedded tissues, the BrdUrd method was compared with tritiated thymidine (3H-TdR) autoradiography, using rat tibiae labelled with both 3H-TdR and BrdUrd, fixed in Carnoy's fluid and decalcified in EDTA, prior to routine paraffin embedding. The distribution of BrdUrd-labelled cells correlated with the sites of cell proliferation in the growing rat tibia. Independent studies with each method on paired serial sections of double-labelled tissue, showed a highly significant correlation (r = 0.81, p less than 0.0003) in the numbers of labelled cells seen in autoradiographs and immunostained sections from the proximal tibial growth plate. Combined BrdUrd immunohistochemistry and 3H-TdR autoradiography showed that the majority of labelled cells in cartilage, bone marrow, and fibrous perichondrium and periosteum had incorporated both labels. These results show that BrdUrd immunohistochemistry is a valid technique for the study of dividing cells in mineralized tissues after decalcification. |
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Authors:
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S S Apte |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Histochemical journal Volume: 22 ISSN: 0018-2214 ISO Abbreviation: Histochem. J. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-12-06 Completed Date: 1990-12-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0163161 Medline TA: Histochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 401-8 Citation Subset: IM |
Affiliation:
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Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Centre, Headington. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography / methods* Bone Marrow / metabolism Bone Marrow Cells* Bromodeoxyuridine / diagnostic use* Cartilage, Articular / cytology*, metabolism Cell Cycle Cell Division DNA / metabolism Growth Plate / cytology*, metabolism Immunohistochemistry / methods* Male Rats Rats, Inbred Strains Thymidine / metabolism Tibia / cytology*, metabolism Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 50-89-5/Thymidine; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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