Document Detail


Validation of Thermally Assisted Hydrolysis and Methylation-Gas Chromatography Using a Vertical Microfurnace Pyrolyzer for the Compositional Analysis of Fatty Acid Components in Microalgae.
MedLine Citation:
PMID:  22458968     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) in the presence of trimethylsulfonium hydroxide, using a vertical microfurnace pyrolyzer, was validated for the compositional analysis of fatty acid components in microalgae. The chromatograms of a microalga Pavlova lutheri obtained under optimized THM conditions clearly showed a series of fatty acid methyl esters including thermally-labile polyunsaturated fatty acid components. Based on these peak areas, their chemical compositions were rapidly determined without using any tedious sample pretreatment with a precision of less than 8% relative standard deviation. Moreover, the compositions thus obtained were in good agreement with those obtained by the conventional technique involving solvent extraction. Finally, the THM-GC technique was applied for the compositional analysis of fatty acid components in a newly found microalga, C. gloeobotrydiformis. The obtained data showed a high abundance (24 mol%) of α-linolenic acid components, suggesting its potential usefulness as feed sources and/or functional foods.
Authors:
Yasuyuki Ishida; Takayuki Honda; Shigemi Mabuchi; Osamu Sueno
Related Documents :
21834238 - Cytotoxicity of active ingredients extracted from plants of the brazilian "cerrado".
21892128 - Bioactivity of carica papaya (caricaceae) against spodoptera frugiperda (lepidoptera: n...
20810648 - Homocitrate synthase connects amino acid metabolism to chromatin functions through esa1...
21946258 - Phospholipase a(2)-susceptible liposomes of anticancer double lipid-prodrugs.
3689438 - Effects of taurocholic acid/hcl alone or after pretreatment with geranylgeranylacetone ...
11038488 - Flow injection system with chemiluminometric detection for enzymatic determination of a...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-30
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chromatographic examination of the chemical composition and sequence distribution of copolymers from...
Next Document:  Functionally Important Amino Acids in Rice Sucrose Transporter OsSUT1.