Document Detail

Validation of Thermally Assisted Hydrolysis and Methylation-Gas Chromatography Using a Vertical Microfurnace Pyrolyzer for the Compositional Analysis of Fatty Acid Components in Microalgae.
MedLine Citation:
PMID:  22458968     Owner:  NLM     Status:  Publisher    
Thermally assisted hydrolysis and methylation-gas chromatography (THM-GC) in the presence of trimethylsulfonium hydroxide, using a vertical microfurnace pyrolyzer, was validated for the compositional analysis of fatty acid components in microalgae. The chromatograms of a microalga Pavlova lutheri obtained under optimized THM conditions clearly showed a series of fatty acid methyl esters including thermally-labile polyunsaturated fatty acid components. Based on these peak areas, their chemical compositions were rapidly determined without using any tedious sample pretreatment with a precision of less than 8% relative standard deviation. Moreover, the compositions thus obtained were in good agreement with those obtained by the conventional technique involving solvent extraction. Finally, the THM-GC technique was applied for the compositional analysis of fatty acid components in a newly found microalga, C. gloeobotrydiformis. The obtained data showed a high abundance (24 mol%) of α-linolenic acid components, suggesting its potential usefulness as feed sources and/or functional foods.
Yasuyuki Ishida; Takayuki Honda; Shigemi Mabuchi; Osamu Sueno
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-30
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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