Document Detail


Validation of Quantitative and Qualitative Methods for Detecting Allergenic Ingredients in Processed Foods in Japan.
MedLine Citation:
PMID:  23039046     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A labeling system for food allergenic ingredients was established in Japan in April 2002. To monitor the labeling, the Japanese government announced the official methods for detecting allergens in processed foods in November 2002. The official methods consist of quantitative screening tests using enzyme-linked immunosorbent assays (ELISAs) and qualitative confirmation tests using western blotting or polymerase chain reactions (PCR). In addition, the Japanese government designated 10 μg protein/g food (the corresponding allergenic ingredient soluble protein weight/food weight), determined by ELISA, as the labeling threshold. To standardize the official methods, the criteria for the validation protocol were described in the official guidelines. This manuscript, which was presented at the Advances in Food Allergen Detection Symposium, ACS National Meeting & Expo, San Diego Spring 2012, describes the validation protocol outlined in the official Japanese guidelines, the results of interlaboratory studies for the quantitative detection method (ELISA for crustacean proteins) and the qualitative detection method (PCR for shrimp and crab DNAs), and the reliability of the detection methods.
Authors:
Shinobu Sakai; Reiko Adachi; Hiroshi Akiyama; Reiko Teshima
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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