| Validation of a postfixation tissue storage and transport medium to preserve histopathology and molecular pathology analyses (total and phosphoactivated proteins, and FISH). | |
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MedLine Citation:
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PMID: 22338055 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tumor biomarker studies are integral to oncology clinical trials but may yield artifactual results owing to variation in sample procurement and processing. Ethanol, 70% vol/vol, was validated as a sample transport medium using markers of the PI3K/Akt/mTOR pathway. BT474 tumor xenografts were excised and slices were immediately placed into formaldehyde and fixed for 24 hours. Fixed tissue slices were immediately processed into paraffin or transferred to 70% vol/vol ethanol and stored at room temperature for 1, 2, and 4 weeks before further processing. Freshly cut tissue sections were evaluated for pAKT(S473), HER2, pHER-2(Y1248), pS6(S235/236), and pS6(S240/244), Ki-67, and HER2 by fluorescence in situ hybridization and stained with H&E and Masson trichrome. No significant changes were observed when comparing samples stored in 70% ethanol for up to 4 weeks with immediately processed tissue. Ethanol, 70% vol/vol, provides a safe storage medium for formaldehyde-fixed tumor tissue, facilitating sample transport during multicenter clinical trials. |
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Authors:
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Michael M Stumm; Maja R Walker; Caroline Stork; Noelle Hanoteau; Urs Wagner; Terence M O'Reilly |
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Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: American journal of clinical pathology Volume: 137 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-02-16 Completed Date: 2012-04-03 Revised Date: 2012-10-12 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 429-36 Citation Subset: AIM; IM |
Affiliation:
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Oncology Research, Novartis Institutes of Biomedical Research, Novartis Pharma AG, Basel, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor DNA / analysis, chemistry Ethanol / chemistry Female Fixatives / chemistry Formaldehyde / chemistry Histocytological Preparation Techniques / methods* Humans In Situ Hybridization, Fluorescence* Mammary Neoplasms, Experimental / genetics, metabolism, pathology Mice Mice, Inbred BALB C Mice, Nude Neoplasm Proteins / metabolism Pathology, Molecular / methods* Phosphatidylinositol 3-Kinases / metabolism Phosphorylation Proto-Oncogene Proteins c-akt / metabolism Receptor, erbB-2 / genetics, metabolism Specimen Handling / methods* Time Factors Tumor Markers, Biological / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fixatives; 0/Neoplasm Proteins; 0/Tumor Markers, Biological; 50-00-0/Formaldehyde; 64-17-5/Ethanol; 9007-49-2/DNA; EC 2.7.1.-/Phosphatidylinositol 3-Kinases; EC 2.7.10.1/ERBB2 protein, human; EC 2.7.10.1/Receptor, erbB-2; EC 2.7.11.1/Proto-Oncogene Proteins c-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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