| Validation of MRI biomarkers of hepatic steatosis in the presence of iron overload in the ob/ob mouse. | |
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MedLine Citation:
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PMID: 22127834 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: To validate the utility and performance of a T 2* correction method for hepatic fat quantification in an animal model of both steatosis and iron overload. MATERIALS AND METHODS: Mice with low (n = 6), medium (n = 6), and high (n = 8) levels of steatosis were sedated and imaged using a chemical shift-based fat-water separation method to obtain magnetic resonance imaging (MRI) fat-fraction measurements. Imaging was performed before and after each of two superparamagnetic iron oxide (SPIO) injections to create hepatic iron overload. Fat-fraction maps were reconstructed with and without T 2* correction. Fat-fraction with and without T 2* correction and T 2* measurements were compared after each injection. Liver tissue was harvested and imaging results were compared to triglyceride extraction and histology grading. RESULTS: Excellent correlation was seen between MRI fat-fraction and tissue-based fat quantification. Injections of SPIOs led to increases in R 2* (=1/T 2*). Measured fat-fraction was unaffected by the presence of iron when T 2* correction was used, whereas measured fat-fraction dramatically increased without T 2* correction. CONCLUSION: Hepatic fat-fraction measured using a T 2*-corrected chemical shift-based fat-water separation method was validated in an animal model of steatosis and iron overload. T 2* correction enables robust fat-fraction estimation in both the presence and absence of iron, and is necessary for accurate hepatic fat quantification. J. Magn. Reson. Imaging 2011;. © 2011 Wiley Periodicals, Inc. |
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Authors:
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Catherine D G Hines; Rashmi Agni; Calista Roen; Ian Rowland; Diego Hernando; Eric Bultman; Debra Horng; Huanzhou Yu; Ann Shimakawa; Jean H Brittain; Scott B Reeder |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-11-29 |
Journal Detail:
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Title: Journal of magnetic resonance imaging : JMRI Volume: - ISSN: 1522-2586 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9105850 Medline TA: J Magn Reson Imaging Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
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Department of Radiology, University of Wisconsin, Madison, Wisconsin, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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