Document Detail


Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam.
MedLine Citation:
PMID:  22774847     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  To evaluate the potential use of MALDI-TOF MS for fast and reliable classification and identification of lactic acid bacteria from traditional fermented foods. Methods and results:  A total of 119 strains of LAB from fermented meat (nem chua) were analyzed with both (GTG)(5) -PCR fingerprinting and MALDI-TOF MS. Cluster analysis of the profiles revealed five species represented by a single isolate both in (GTG)(5) -PCR and in MALDI-TOF MS; five species grouped alike for (GTG)(5) -PCR and for MALDI-TOF MS, however, differences in minimal similarity between the delineated (GTG)(5) -PCR and MALDI-TOF MS clusters could be observed; three species showed more heterogeneity in their MALDI-TOF MS profiles compared to their (GTG)(5) -PCR profiles; two species, each represented by a single MALDI-TOF cluster were subdivided in the corresponding (GTG)(5) -PCR dendrogram. As proof of the identification potential of MALDI-TOF MS, LAB diversity from one fermented mustard sample was analyzed using MALDI- TOF MS. PheS gene sequencing was used for validation. Conclusions:  MALDI-TOF MS is a powerful, fast, reliable, and cost effective technique for the identification of lactic acid bacteria (LAB) associated with the production of fermented foods. Significance and Impact of the Study:  Food LAB can be identified using MALDI-TOF MS and its application could possibly be extended to other food matrices and/or other food derived micro-organisms. © 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Doan Thi Lam Nguyen; Koenraad Van Hoorde; Margo Cnockaert; Evie De Brandt; Maarten Aerts; Binh Le Thanh; Peter Vandamme
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-9
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Faculty of Applied Bioscience Engineering, University College Ghent, Valentin Vaerwyckweg 1, 9000 Gent, Belgium Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam Department of Biochemistry and Food Biotechnology, Faculty of Food Science and Technology, Hanoi University of Agriculture, Trauquy-Gialam, Hanoi, Vietnam.
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