| Validation of an LC-ESI-MS/MS method for the quantitation of phosphodiesterase-5 inhibitors and their main metabolites in rat serum and brain tissue samples. | |
| | |
MedLine Citation:
|
PMID: 22647499 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
This work proposes a liquid chromatography-electrospray ionization ion trap mass spectrometry (LC-ESI-ITMS) method, for the quantification of sildenafil (SDF), tadalafil (TDF) and vardenafil (VDF) and their metabolites N-desmethylSDF, O-desethylSDF and N-desethylVDF, preceded by a sample preparation step based on protein and phospholipid elimination. A C8 column (150mm×4.6mm, 5μm) with ammonium formate (20mM) and acetonitrile as the mobile phase components have been used. This method has been validated, obtaining limits of quantification ranged from 1 to 2.5ng/mL and 2 to 5ng/g in serum and brain tissue respectively, while limits of detection ranged from 0.3 to 0.9ng/mL in serum and 0.6 to 1.9ng/g in brain tissue. Assay recoveries for low level QC samples were higher than 83% and the matrix effect ranged between 91% and 108% in serum and between 98% and 107% in brain tissue. The method has been applied to the quantification of these compounds in the serum and brain tissue of rats treated intraperitoneally with 10mg/kg of SDF, TDF or VDF. |
| | |
Authors:
|
Nora Unceta; Leire Echeazarra; Mario Montaña; Joan Sallés; Alberto Gómez-Caballero; M Aránzazu Goicolea; Ramón J Barrio |
Related Documents
:
|
18204189 - Ion mobility spectrometry in the diagnosis of sarcoidosis: results of a feasibility study. 22503209 - Magnetic beads-based enzymatic spectrofluorometric assay for rapid and sensitive detect... 8249559 - Evaluation of a new system for ventilatory administration of nitric oxide. 17361389 - Determination of functional residual capacity by oxygen washin-washout: a validation st... 22666159 - Trace level arsenic quantification through cloud point extraction: application to biolo... 22489549 - Determination of tricyclazole content in paddy rice by surface enhanced raman spectrosc... 11688649 - On-line mercury and methylmercury pre-concentration by adsorption of their dithiophosph... 18558109 - Determination of pesticides in honey using excitation-emission matrix fluorescence coup... 17221929 - Oxygen-18 measurement of andalusian olive oils by continuous flow pyrolysis/isotope rat... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-5-10 |
Journal Detail:
|
Title: Journal of pharmaceutical and biomedical analysis Volume: - ISSN: 1873-264X ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-5-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
|
Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cysteinyl leukotriene receptors in tonsillar B- and T-lymphocytes from children with obstructive sle...
Next Document: Sand box experiments with bioclogging of porous media: Hydraulic conductivity reductions.