Document Detail


Validation of the ANSR Salmonella method for detection of Salmonella spp. in selected foods and environmental samples.
MedLine Citation:
PMID:  24000759     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
ANSR Salmonella is a new molecular diagnostic assay for detection of Salmonella spp. in foods and environmental samples. The test is based on the nicking enzyme amplification reaction (NEAR) isothermal nucleic acid amplification technology. The assay platform features simple instrumentation, minimal labor, and, following a single-step 10-24 h enrichment (depending on sample type), an extremely short assay time of 30 min, including sample preparation. Detection is real-time using fluorescent molecular beacon probes. Inclusivity testing was performed using a panel of 113 strains of S. enterica and S. bongori, representing 109 serovars and all genetic subgroups. With the single exception of the rare serovar S. Weslaco, all serovars and genetic subgroups were detected. Exclusivity testing of 38 non-salmonellae, mostly Enterobacteriaceae, yielded no evidence of cross-reactivity. In comparative testing of chicken carcass rinse, raw ground turkey, raw ground beef, hot dogs, and oat cereal, there were no statistically significant differences in the number of positive results obtained with the ANSR and the U.S. Department of Agriculture-Food Safety and Inspection Service or U.S. Food and Drug Administration/Bacteriological Analytical Manual reference culture methods. In testing of swab or sponge samples from five different environmental surfaces, four trials showed no statistically significant differences in the number of positive results by the ANSR and the U.S. Food and Drug Administration/ Bacteriological Analytical Manual reference methods; in the trial with stainless steel surface, there were significantly more positive results by the ANSR method. Ruggedness experiments showed a high degree of assay robustness when deviations in reagent volumes and incubation times were introduced.
Authors:
Mark Mozola; Paul Norton; Susan Alles; R Lucas Gray; Jerry Tolan; Oscar Caballero; Lisa Pinkava; Edan Hosking; Karen Luplow; Jennifer Rice
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  96     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2013 Jul-Aug
Date Detail:
Created Date:  2013-09-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  842-53     Citation Subset:  IM    
Affiliation:
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA. mmozola@neogen.com
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