| Validated method for L-ornithine-L-aspartate analysis in human plasma by capillary electrophoresis. | |
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MedLine Citation:
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PMID: 16078310 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A rapid capillary electrophoresis method for routine determination of two amino acids, L-ornithine and L-aspartic acid, in human plasma is reported. The method runs automatically, requires a minimum of sample preparation and moreover includes no extensive extraction and no gradient or derivatization procedure. Analyses were performed on an uncoated silica capillary using buffer solution composed with 10 mM sodium tetraborate and 1 M sodium hydroxide (pH=10.0). A capillary electrophoresis P/ACE system equipped with UV detection (200 nm), an automatic injector, a fluid cooled cartridge and System Gold data station was used in this study. The total analysis time under these conditions was 8.0 min. The calibration curve was linear in the range 10-280 microg mL-1 for L-aspartic acid and 20-280 microg mL-1 for L-ornithine (for both amino acids, r=0.999). The method was validated by inaccuracy (bias) and precision (RSD) studies by analysing samples. The method was successfully applied to the quantitative determination of L-ornithine-L-aspartate in human plasma and could be useful for clinical and bioavailability investigations. |
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Authors:
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P Kowalski; M Bieniecki; I Oledzka; H Lamparczyk |
Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 20 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-24 Completed Date: 2006-03-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: England |
Other Details:
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Languages: eng Pagination: 185-94 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2005 John Wiley & Sons, Ltd. |
Affiliation:
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Medical University of Gdańsk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdańsk, Poland. piotrpl@wp.pl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspartic Acid
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blood* Dipeptides / blood* Drug Stability Electrophoresis, Capillary / methods* Freezing Humans Ornithine / blood* Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 3230-94-2/ornithylaspartate; 56-84-8/Aspartic Acid; 7006-33-9/Ornithine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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