Document Detail


Validated PCR assay for the routine detection of Salmonella in food.
MedLine Citation:
PMID:  16496566     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed a rapid and reliable PCR assay with genus-specific primers for the detection of Salmonella in food samples. With these primers, no primer-specific amplicons were detected when challenged with cultures of microorganisms other than salmonellae, and positive results, i.e., Salmonella-specific bands, were obtained with pure cultures of all 125 Salmonella isolates tested, which represented 100 serovars. The PCR assay was optimized using both pure cultures and artificially inoculated food samples. The assay results were compared with those of the Australian standard culture methods, using more than 500 "naturally" contaminated food samples, over a period of 9 years. Food samples were subjected to nonselective preenrichment in buffered peptone water followed by selective enrichment in Rappaport Vassiliadis (RV) broth and mannitol selenite cystine (MSC) broth. A simple sample preparation method was developed based on concentrating bacterial cells from 1 ml of RV or MSC broths. The PCR results were in perfect agreement with the results of the standard culture methods; no false-positive or false-negative results were obtained. However, the PCR assay was extremely rapid, and results could be obtained within 4 h of testing of enrichment broths.
Authors:
N S Bansal; V Gray; F McDonell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  69     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-24     Completed Date:  2006-03-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  282-7     Citation Subset:  IM    
Affiliation:
Institute of Clinical Pathology and Medical Research, Division of Analytical Laboratories, Western Sydney Area Health Services, Lidcombe, New South Wales, Australia. nbansal@dal.wsahs.nsw.gov.au
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MeSH Terms
Descriptor/Qualifier:
Australia
Colony Count, Microbial / methods
Consumer Product Safety
DNA Primers
Food Contamination / analysis*
Food Microbiology*
Humans
Polymerase Chain Reaction / methods*,  standards*
Reproducibility of Results
Salmonella / isolation & purification*
Sensitivity and Specificity
Species Specificity
Time Factors
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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