Document Detail


Vaginal Lactobacillus microbiota of healthy women in the late first trimester of pregnancy.
MedLine Citation:
PMID:  17877778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: This study was undertaken to characterise the dominant species of Lactobacillus colonising the vagina of healthy pregnant women, to examine some of their phenotypic and genotypic properties, and to gain a better understanding of the potential role of species, which might be associated with infection-free status. DESIGN: A prospective descriptive cohort study. SETTING: Department of Obstetrics and Gynaecology, Medical University of Vienna and Medical School, Vienna, Austria. SAMPLE: A total of 200 women in the late first trimester of pregnancy without clinical signs of vaginal infection were included in the study. Of these, 126 women were found to have a normal vaginal flora based on Gram stain. METHODS: Culture probes from those 126 women were further processed for identification of Lactobacillus species. Overall, 168 colonies from 84 women were identified as belonging to the Lactobacillus genus. Based on the combined results of microbiological methods and genus-specific, multiplex, and species-specific polymerase chain reaction, lactobacilli were recovered from 72 women. MAIN OUTCOME MEASURES: Identification of Lactobacillus species of the vaginal flora of healthy pregnant women. RESULTS: The most frequently occurring species were Lactobacillus crispatus and Lactobacillus gasseri, followed by Lactobacillus jensenii and Lactobacillus rhamnosus. CONCLUSIONS: Our results may have implications on the composition and on the use of Lactobacillus preparations for the prevention of recurrent vaginal infection.
Authors:
H Kiss; B Kögler; L Petricevic; I Sauerzapf; S Klayraung; K Domig; H Viernstein; W Kneifel
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Publication Detail:
Type:  Journal Article     Date:  2007-09-17
Journal Detail:
Title:  BJOG : an international journal of obstetrics and gynaecology     Volume:  114     ISSN:  1471-0528     ISO Abbreviation:  BJOG     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-22     Completed Date:  2007-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100935741     Medline TA:  BJOG     Country:  England    
Other Details:
Languages:  eng     Pagination:  1402-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics and Gynecology, Medical University and Medical School of Vienna, Vienna, Austria. herbert.kiss@meduniwien.ac.at
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cohort Studies
Female
Genotype
Humans
Lactobacillus / genetics,  isolation & purification*
Phenotype
Polymerase Chain Reaction / methods
Pregnancy / physiology*
Pregnancy Trimester, First
Prospective Studies
Vagina / microbiology*
Vaginal Smears / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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