Document Detail

Vaginal Canine Transmissible Venereal Tumour Associated with Intra-tumoural Leishmania spp. Amastigotes in an Asymptomatic Female Dog.
MedLine Citation:
PMID:  23348016     Owner:  NLM     Status:  Publisher    
A 2-year-old female boxer dog was presented with a vaginal serosanguineous discharge not associated with oestrus. There was a friable mass occupying the upper caudal part of the vagina. Cytological and histological examination revealed a monomorphic population of neoplastic round cells consistent with canine transmissible venereal tumour (TVT). In addition, Leishmania spp. amastigotes were found within the neoplastic tissue. In order to characterize whether the amastigotes were present inside macrophages and/or neoplastic cells, a co-localization study using cell- and pathogen-specific markers was performed. To detect Leishmania spp. a 5.8S ribosomal RNA (rRNA) parasite-specific sequence was used for in-situ hybridization and Mac387 was used as a macrophage marker for immunohistochemistry. Leishmania spp. rRNA was detected inside Mac387(+) macrophages and within the cytoplasm of some neoplastic cells. DNA isolation and polymerase chain reaction using specific primers and sequencing analysis identified the organism as Leishmania infantum (syn. Leishmania chagasi). This is the first report describing infection of tumour cells by L. infantum in a genital TVT from an asymptomatic bitch. Transplantation of Leishmania-laden neoplastic cells could represent an alternative route of venereal transmission of leishmaniasis among dogs.
K Kegler; A Habierski; K Hahn; S P Amarilla; F Seehusen; W Baumgärtner
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-21
Journal Detail:
Title:  Journal of comparative pathology     Volume:  -     ISSN:  1532-3129     ISO Abbreviation:  J. Comp. Pathol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0102444     Medline TA:  J Comp Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Pathology, University of Veterinary Medicine, Bünteweg 2, D-30559 Hannover, Germany; Department of Pathology, Faculty of Veterinary Medicine, National University of Asuncion, Ruta Mcal. Estigarribia km 10, San Lorenzo, Paraguay.
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