Document Detail

Vacuum-ultraviolet circular dichroism reveals DNA duplex formation between short strands of adenine and thymine.
MedLine Citation:
PMID:  23034556     Owner:  NLM     Status:  Publisher    
Absorbance spectroscopy is used extensively to tell when two DNA single strands come together and form a double strand. Here we show that circular dichroism in the vacuum ultraviolet region provides an even stronger indication for duplex formation in the case of short strands of adenine and thymine (4 to 16 bases in each strand). Indeed, our results show that a strong positive CD band appears at 179 nm when double strands are formed. Melting experiments were done in aqueous solution with and without added Na(+) counter ions. With additional salt present a huge increase in the 179 nm CD band was observed when lowering the temperature. A 179 nm CD marker band for duplex formation can be used to measure the kinetics for the association of two single strands. Such experiments rely on large changes at one particular wavelength since it is too time-consuming to record a full-wavelength spectrum.
Lisbeth Munksgaard Nielsen; Søren Vrønning Hoffmann; Steen Brøndsted Nielsen
Related Documents :
9087626 - Adenosine stimulation of dna synthesis in human endothelial cells.
17319276 - Anticlastogenic effect of redistilled cow's urine distillate in human peripheral lympho...
698226 - Effect of l-ethionine on macromolecular synthesis in mitogen-stimulated lymphocytes.
1813386 - Regulation of human lymphocyte proliferation by a tumour cell-derived dna fraction.
16195216 - Age-related changes in oxidative dna damage and benzo(a)pyrene diolepoxide-i (bpde-i)-d...
19321146 - Innate immunity: cytoplasmic dna sensing by the aim2 inflammasome.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Physical chemistry chemical physics : PCCP     Volume:  -     ISSN:  1463-9084     ISO Abbreviation:  Phys Chem Chem Phys     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888160     Medline TA:  Phys Chem Chem Phys     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  [Pedlar inexpensive ergometer-based exercise program improves activity intolerance in a heart failur...
Next Document:  Elderly men with renal dysfunction are most at risk for poor outcome after neck of femur fractures.