Document Detail

Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl(-) and water.
MedLine Citation:
PMID:  11728476     Owner:  NLM     Status:  MEDLINE    
The protein vacuolating toxin A (VacA) of Helicobacter pylori converts late endosomes into large vacuoles in the presence of permeant bases. Here it is shown that this phenomenon corresponds to an accumulation of permeant bases and Cl(-) in HeLa cells and requires the presence of extracellular Cl(-). The net influx of Cl(-) is due to electroneutral, Na(+), K(+), 2Cl(-) cotransporter-mediated transport. Cell vacuolation leads to cell volume increase, consistent with water flux into the cell, while hyper-osmotic media decreased vacuole formation. These data represent the first evidence that VacA-treated cells undergo an osmotic unbalance, reinforcing the hypothesis that the VacA chloride channel is responsible for cell vacuolation.
L Morbiato; F Tombola; S Campello; G Del Giudice; R Rappuoli; M Zoratti; E Papini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  508     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-30     Completed Date:  2001-12-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  479-83     Citation Subset:  IM    
Centro CNR Biomembrane, Department of Biomedical Sciences, University of Padova, Padua, Italy.
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MeSH Terms
Ammonium Chloride / pharmacology
Bacterial Proteins / metabolism,  toxicity*
Bumetanide / pharmacology
Cell Membrane Permeability
Cell Size
Chlorides / metabolism*
Endosomes / metabolism
Hela Cells
Helicobacter pylori*
Hydrogen-Ion Concentration
Methylamines / metabolism
Osmotic Pressure
Sodium-Potassium-Chloride Symporters / antagonists & inhibitors,  metabolism
Vacuoles / drug effects,  metabolism*
Water / metabolism*
Reg. No./Substance:
0/Bacterial Proteins; 0/Chlorides; 0/Methylamines; 0/Sodium-Potassium-Chloride Symporters; 0/VacA protein, Helicobacter pylori; 12125-02-9/Ammonium Chloride; 28395-03-1/Bumetanide; 74-89-5/methylamine; 7732-18-5/Water

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