| Vacuolation induced by VacA toxin of Helicobacter pylori requires the intracellular accumulation of membrane permeant bases, Cl(-) and water. | |
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MedLine Citation:
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PMID: 11728476 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The protein vacuolating toxin A (VacA) of Helicobacter pylori converts late endosomes into large vacuoles in the presence of permeant bases. Here it is shown that this phenomenon corresponds to an accumulation of permeant bases and Cl(-) in HeLa cells and requires the presence of extracellular Cl(-). The net influx of Cl(-) is due to electroneutral, Na(+), K(+), 2Cl(-) cotransporter-mediated transport. Cell vacuolation leads to cell volume increase, consistent with water flux into the cell, while hyper-osmotic media decreased vacuole formation. These data represent the first evidence that VacA-treated cells undergo an osmotic unbalance, reinforcing the hypothesis that the VacA chloride channel is responsible for cell vacuolation. |
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Authors:
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L Morbiato; F Tombola; S Campello; G Del Giudice; R Rappuoli; M Zoratti; E Papini |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 508 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-11-30 Completed Date: 2001-12-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 479-83 Citation Subset: IM |
Affiliation:
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Centro CNR Biomembrane, Department of Biomedical Sciences, University of Padova, Padua, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ammonium Chloride
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pharmacology Bacterial Proteins / metabolism, toxicity* Bumetanide / pharmacology Cell Membrane Permeability Cell Size Chlorides / metabolism* Endosomes / metabolism Hela Cells Helicobacter pylori* Humans Hydrogen-Ion Concentration Methylamines / metabolism Osmotic Pressure Sodium-Potassium-Chloride Symporters / antagonists & inhibitors, metabolism Vacuoles / drug effects, metabolism* Water / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Chlorides; 0/Methylamines; 0/Sodium-Potassium-Chloride Symporters; 0/VacA protein, Helicobacter pylori; 12125-02-9/Ammonium Chloride; 28395-03-1/Bumetanide; 74-89-5/methylamine; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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