Document Detail

Vaccinia virus temperature-sensitive mutants in the A28 gene produce non-infectious virions that bind to cells but are defective in entry.
MedLine Citation:
PMID:  17499330     Owner:  NLM     Status:  MEDLINE    
The vaccinia virus temperature-sensitive mutations Cts6 and Cts9 were mapped by marker rescue and DNA sequencing to the A28 gene. Cts6 and Cts9 contain an identical 2-bp deletion truncating the A28 protein and removing the fourth conserved cysteine near the C-terminus. Cts9 mutant virions produced at 40 degrees C were non-infectious and unable to cause cytopathic effect. However, the mutant A28 protein localized to purified mature virions (MV) at 31 degrees C and 40 degrees C. MV of Cts9 produced at 40 degrees C bound to cells but did not enter cells. Low pH treatment of Cts9-infected cells at 18 h p.i. failed to produce fusion from within at 40 degrees C, but gave fusion at 31 degrees C. Adsorption of Cts9 mutant virions to cells followed by low pH treatment showed a defect in fusion from without. The Cts9 phenotype suggests that the A28 protein is involved in both virus entry and cell-cell fusion, and supports the linkage between the two processes.
Peter C Turner; Bradley P Dilling; Cindy Prins; Steven G Cresawn; Richard W Moyer; Richard C Condit
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-17
Journal Detail:
Title:  Virology     Volume:  366     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-03     Completed Date:  2007-11-13     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  62-72     Citation Subset:  IM    
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MeSH Terms
3' Untranslated Regions
Cell Fusion
Cell Line
Cercopithecus aethiops
DNA Viruses / genetics,  pathogenicity,  physiology
Genes, Viral*
Genome, Viral
Recombination, Genetic
Vaccinia virus / genetics*,  immunology,  pathogenicity*,  physiology
Viral Vaccines
Virion / genetics,  physiology
Grant Support
R01 AI015722-24/AI/NIAID NIH HHS; R01 AI055560/AI/NIAID NIH HHS; R01 AI055560-01A1/AI/NIAID NIH HHS; R01 AI055560-02/AI/NIAID NIH HHS; R01 AI055560-03/AI/NIAID NIH HHS; R01 AI055560-04/AI/NIAID NIH HHS; R01 AI055560-05/AI/NIAID NIH HHS; R21 AI055560/AI/NIAID NIH HHS; R21 AI055560-01/AI/NIAID NIH HHS
Reg. No./Substance:
0/3' Untranslated Regions; 0/Viral Vaccines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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