| Vaccinia virus temperature-sensitive mutants in the A28 gene produce non-infectious virions that bind to cells but are defective in entry. | |
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MedLine Citation:
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PMID: 17499330 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The vaccinia virus temperature-sensitive mutations Cts6 and Cts9 were mapped by marker rescue and DNA sequencing to the A28 gene. Cts6 and Cts9 contain an identical 2-bp deletion truncating the A28 protein and removing the fourth conserved cysteine near the C-terminus. Cts9 mutant virions produced at 40 degrees C were non-infectious and unable to cause cytopathic effect. However, the mutant A28 protein localized to purified mature virions (MV) at 31 degrees C and 40 degrees C. MV of Cts9 produced at 40 degrees C bound to cells but did not enter cells. Low pH treatment of Cts9-infected cells at 18 h p.i. failed to produce fusion from within at 40 degrees C, but gave fusion at 31 degrees C. Adsorption of Cts9 mutant virions to cells followed by low pH treatment showed a defect in fusion from without. The Cts9 phenotype suggests that the A28 protein is involved in both virus entry and cell-cell fusion, and supports the linkage between the two processes. |
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Authors:
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Peter C Turner; Bradley P Dilling; Cindy Prins; Steven G Cresawn; Richard W Moyer; Richard C Condit |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-17 |
Journal Detail:
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Title: Virology Volume: 366 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-09-03 Completed Date: 2007-11-13 Revised Date: 2013-03-05 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 62-72 Citation Subset: IM |
Affiliation:
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Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, FL 32610-0266, USA. pturner@mgm.ufl.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3' Untranslated Regions Animals Cell Fusion Cell Line Cercopithecus aethiops DNA Viruses / genetics, pathogenicity, physiology Genes, Viral* Genome, Viral Haplorhini Mutation* Recombination, Genetic Temperature Vaccinia virus / genetics*, immunology, pathogenicity*, physiology Viral Vaccines Virion / genetics, physiology |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI015722-24/AI/NIAID NIH HHS; R01 AI055560/AI/NIAID NIH HHS; R01 AI055560-01A1/AI/NIAID NIH HHS; R01 AI055560-02/AI/NIAID NIH HHS; R01 AI055560-03/AI/NIAID NIH HHS; R01 AI055560-04/AI/NIAID NIH HHS; R01 AI055560-05/AI/NIAID NIH HHS; R01 AI055560-08/AI/NIAID NIH HHS; R21 AI055560-01/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/3' Untranslated Regions; 0/Viral Vaccines |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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