| Vaccination with recombinant adhesins from the RgpA-Kgp proteinase-adhesin complex protects against Porphyromonas gingivalis infection. | |
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MedLine Citation:
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PMID: 16839648 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Porphyromonas gingivalis is a pathogen associated with chronic periodontitis, an inflammatory disease of the supporting tissues of the teeth. A major virulence factor for P. gingivalis is the RgpA-Kgp proteinase-adhesin complex. We have prepared the following recombinant proteins corresponding to domains/regions of the RgpA-Kgp complex; rRgpA(cat), rRgpA(A1), rRgpA(A2), rRgpA(A4), rRgpA(A1)(784-1009), rRgpA(A1)(911-1009), rKgp(A1) and rKgp(A1)(759-989). The ability of each recombinant protein to attenuate P. gingivalis infection, when used as a vaccine, in the murine lesion model was determined. All of the recombinant adhesin domains were found to significantly attenuate P. gingivalis infection with the most effective being rKgp(A1) and rKgp(A1)(759-989) followed by rRgpA(A1), rRgpA(A1)(784-1009) and rRgpA(A1)(911-1009). The predominant antibody isotype was IgG1. The A1 adhesins, which gave the best protection, contain specific motifs implicated in binding to host tissue. Immunisation with rRgpA(cat) had no effect on P. gingivalis infection. As well as detecting the Kgp(A1) adhesin in a Western blot, the rKgp(A1) and rKgp(A1)(759-989) antisera were also immunoreactive with the A1 and A3 adhesins of RgpA and HagA. Flow cytometric analysis indicated that rKgp(A1) and rRgpA(A1) antisera recognised native antigen on P. gingivalis whole cells. Furthermore, the rKgp(A1) and rKgp(A1)(759-989) antisera exhibited a similar immunoreactive profile with outer membrane preparations of all P. gingivalis serotypes and clinical isolates tested. The recombinant A1 adhesin therefore has potential in the development of a P. gingivalis vaccine. |
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Authors:
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Leanne T Frazer; Neil M O'Brien-Simpson; Nada Slakeski; Katrina A Walsh; Paul D Veith; Chao Guang Chen; Ian G Barr; Eric C Reynolds |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2006-06-23 |
Journal Detail:
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Title: Vaccine Volume: 24 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-11 Completed Date: 2006-12-01 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 6542-54 Citation Subset: IM |
Affiliation:
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Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne, 720 Swanston Street, Victoria 3010, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adhesins, Bacterial
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immunology* Animals Bacterial Outer Membrane Proteins / immunology Bacterial Vaccines / immunology* Bacteroidaceae Infections / immunology*, prevention & control* Blotting, Western Catalysis Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Flow Cytometry Immunization Mice Mice, Inbred BALB C Peptide Hydrolases / immunology Peptide Mapping Porphyromonas gingivalis / immunology* Vaccines, Synthetic / immunology |
| Grant Support | |
ID/Acronym/Agency:
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1R01 DE14198-01/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adhesins, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Vaccines; 0/Vaccines, Synthetic; EC 3.4.-/Peptide Hydrolases |
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