Document Detail

VIP receptors in pig liver cells: binding characteristics and role in degradation.
MedLine Citation:
PMID:  6089136     Owner:  NLM     Status:  MEDLINE    
The physiological role of VIP in the liver is controversial. VIP receptors are present, but their function in the metabolic regulation is uncertain. The interaction of porcine VIP with isolated cells from pig liver was studied with respect to receptor-binding, degradation and glycogenolytic action. In this model, VIP and liver showed homology of animal species. 1. Receptor-binding was heterogenous with Kd values of 10(-9) mol/l and 4 X 10(-8) mol/l, and a total amount of binding sites of 7 X 10(-11) mol per 10(9) cells. The peptide specificity showed that porcine and chicken VIP were equally potent in inhibiting receptor-bound 125I-VIP; secretin was about 30 times less potent; glucagon and somatostatin were ineffective. 2. Receptor-bound 125I-VIP was degraded since about 70% was released as radioactivity not reacting with VIP-antiserum. 3. Glucose-release was not stimulated by VIP (10(-6) mol/l) whereas the rate was increased two-fold by glucagon (10(-6) mol/l). In conclusion, VIP receptors in pig liver cells are different from other tissues regarding peptide specificity. It is suggested that receptor-binding mediates degradation of VIP by pig liver rather than metabolic effects.
S Gammeltoft; P Staun-Olsen; B Ottesen; J Fahrenkrug
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Peptides     Volume:  5     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:    1984 Mar-Apr
Date Detail:
Created Date:  1984-09-24     Completed Date:  1984-09-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  367-70     Citation Subset:  IM    
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MeSH Terms
Glucagon / pharmacology
Glucose / metabolism
Liver / drug effects,  metabolism*
Receptors, Cell Surface / metabolism*
Receptors, Vasoactive Intestinal Peptide
Vasoactive Intestinal Peptide / metabolism*,  pharmacology
Reg. No./Substance:
0/Receptors, Cell Surface; 0/Receptors, Vasoactive Intestinal Peptide; 37221-79-7/Vasoactive Intestinal Peptide; 50-99-7/Glucose; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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