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VEGF expression and microvascular responses to severe transient hypoxia in the fetal sheep brain.
MedLine Citation:
PMID:  23222909     Owner:  NLM     Status:  Publisher    
Background:Fetal hypoxia contributes significantly to the pathogenesis of permanent perinatal brain injury. We hypothesized that hypoxia-induced cerebral angiogenesis and microvascular changes would occur in fetal sheep subjected to a severe hypoxic insult produced by occluding the umbilical cord (UCO) for 10 mins.Methods:At 124-126 days gestation singleton fetal sheep underwent surgery for implantation of catheters and placement of an inflatable cuff around the umbilical cord. A 10 mins UCO or sham UCO (n=5) was induced at 132 days gestation. The fetal brain was collected at 24 h (n=5) or 48 h (n=4) after UCO for immunohistochemical analysis of VEGF, Ki67 and serum albumin.Results:By 48 h after UCO, the percentage of blood vessels expressing VEGF had increased in the subventricular zone, periventricular and subcortical white matter, corpus callosum and cortex. Alterations in vascular permeability (albumin extravasation) were observed only in periventricular and subcortical white matter, and in the subventricular zone following UCO.Conclusion:The up-regulation of VEGF expression and increased leakage of plasma protein in the fetal sheep brain shows that the microvasculature in white matter is sensitive to hypoxia in the near term brain.Pediatric Research (2012); doi:10.1038/pr.2012.191.
Ana A Baburamani; Margie Castillo-Melendez; David W Walker
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-10
Journal Detail:
Title:  Pediatric research     Volume:  -     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1] The Ritchie Centre, Monash Institute of Medical Research, Monash University, Clayton, Victoria, 3800, Australia [2] Department of Physiology, Monash University, Clayton, Victoria, 3800, Australia.
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