| VEGF is critical for spontaneous differentiation of stem cells into cardiomyocytes. | |
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MedLine Citation:
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PMID: 17276389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cardiomyocyte regeneration is limited in adult life and is not sufficient to compensate for cell loss with myocardial infarction. Hence, the identification of a useful source of cardiomyocyte progenitors is of great interest for possible use in regenerative therapy. In this study, we isolated stem cells derived from human subcutaneous adipose tissue. The expression of Nkx2.5 and GATA-4 can be observed by PCR directly after extraction and during cultivation in some of these cells. Cardiac Troponin T and myosin light chain-2v become positive after 12 days of cultivation. To define respective factors responsible for spontaneous differentiation, we measured VEGF level in ADSC conditioned medium. Our data showed that ADSC secrete significant amount of VEGF (283.5pg per microgram DNA) and that anti-VEGF receptor antibodies blocked the cardiac differentiation. In conclusion, we demonstrated the spontaneous differentiation of human subcutaneous adipose-derived stem cells into a cardiomyocyte phenotype under standard culturing conditions. |
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Authors:
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Yao-Hua Song; Sebastian Gehmert; Sanga Sadat; Kai Pinkernell; Xiaowen Bai; Nadine Matthias; Eckhard Alt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-25 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 354 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-13 Completed Date: 2007-04-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 999-1003 Citation Subset: IM |
Affiliation:
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Department of Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cardiac Myosins / biosynthesis Cell Differentiation / physiology* Cells, Cultured GATA4 Transcription Factor / biosynthesis Homeodomain Proteins / biosynthesis Humans Myocytes, Cardiac / cytology*, physiology* Myosin Light Chains / biosynthesis Rats Stem Cells / physiology* Subcutaneous Fat / cytology Transcription Factors / biosynthesis Troponin T / biosynthesis Vascular Endothelial Growth Factor A / physiology* |
| Chemical | |
Reg. No./Substance:
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0/GATA4 Transcription Factor; 0/Homeodomain Proteins; 0/Myosin Light Chains; 0/Transcription Factors; 0/Troponin T; 0/Vascular Endothelial Growth Factor A; 0/myosin light chain 2; EC 3.6.1.-/Cardiac Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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