Document Detail

VEGF is critical for spontaneous differentiation of stem cells into cardiomyocytes.
MedLine Citation:
PMID:  17276389     Owner:  NLM     Status:  MEDLINE    
Cardiomyocyte regeneration is limited in adult life and is not sufficient to compensate for cell loss with myocardial infarction. Hence, the identification of a useful source of cardiomyocyte progenitors is of great interest for possible use in regenerative therapy. In this study, we isolated stem cells derived from human subcutaneous adipose tissue. The expression of Nkx2.5 and GATA-4 can be observed by PCR directly after extraction and during cultivation in some of these cells. Cardiac Troponin T and myosin light chain-2v become positive after 12 days of cultivation. To define respective factors responsible for spontaneous differentiation, we measured VEGF level in ADSC conditioned medium. Our data showed that ADSC secrete significant amount of VEGF (283.5pg per microgram DNA) and that anti-VEGF receptor antibodies blocked the cardiac differentiation. In conclusion, we demonstrated the spontaneous differentiation of human subcutaneous adipose-derived stem cells into a cardiomyocyte phenotype under standard culturing conditions.
Yao-Hua Song; Sebastian Gehmert; Sanga Sadat; Kai Pinkernell; Xiaowen Bai; Nadine Matthias; Eckhard Alt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-25
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  354     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-04-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  999-1003     Citation Subset:  IM    
Department of Molecular Pathology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
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MeSH Terms
Cardiac Myosins / biosynthesis
Cell Differentiation / physiology*
Cells, Cultured
GATA4 Transcription Factor / biosynthesis
Homeodomain Proteins / biosynthesis
Myocytes, Cardiac / cytology*,  physiology*
Myosin Light Chains / biosynthesis
Stem Cells / physiology*
Subcutaneous Fat / cytology
Transcription Factors / biosynthesis
Troponin T / biosynthesis
Vascular Endothelial Growth Factor A / physiology*
Reg. No./Substance:
0/GATA4 Transcription Factor; 0/Homeodomain Proteins; 0/Myosin Light Chains; 0/Transcription Factors; 0/Troponin T; 0/Vascular Endothelial Growth Factor A; 0/myosin light chain 2; EC 3.6.1.-/Cardiac Myosins

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