Document Detail


VDAC1 serves as a mitochondrial binding site for hexokinase in oxidative muscles.
MedLine Citation:
PMID:  17207767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Voltage-dependent anion channels (VDACs), also known as mitochondrial porins, are the main pathway for metabolites across the mitochondrial outer membrane and may serve as binding sites for kinases, including hexokinase. We determined that mitochondria-bound hexokinase activity is significantly reduced in oxidative muscles (heart and soleus) in vdac1(-/-) mice. The activity data were supported by western blot analysis using HK2 specific antibody. To gain more insight into the physiologic mean of the results with the activity data, VDAC deficient mice were subjected to glucose tolerance testing and exercise-induced stress, each of which involves tissue glucose uptake via different mechanisms. vdac1(-/-) mice exhibit impaired glucose tolerance whereas vdac3(-/-) mice have normal glucose tolerance and exercise capacity. Mice lacking both VDAC1 and VDAC3 (vdac1(-/-)/vdac3(-/-)) have reduced exercise capacity together with impaired glucose tolerance. Therefore, we demonstrated a link between VDAC1 mediated mitochondria-bound hexokinase activity and the capacity for glucose clearance.
Authors:
Keltoum Anflous-Pharayra; Zong-Jin Cai; William J Craigen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-11-23
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1767     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-27     Completed Date:  2007-04-10     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  136-42     Citation Subset:  IM    
Affiliation:
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA. ayapharayra@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Blotting, Western
Female
Glucose Tolerance Test
Hexokinase / metabolism*
Male
Mice
Mitochondria, Muscle / metabolism*
Mitochondrial Proteins / deficiency
Motor Activity / physiology
Muscle, Skeletal / metabolism
Myocardium / metabolism
Voltage-Dependent Anion Channel 1 / deficiency,  physiology*
Voltage-Dependent Anion Channels / deficiency
Grant Support
ID/Acronym/Agency:
R01 GM55713/GM/NIGMS NIH HHS; R01 NS42319/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Mitochondrial Proteins; 0/Vdac1 protein, mouse; 0/Vdac3 protein, mouse; 0/Voltage-Dependent Anion Channels; EC 1.6.-/Voltage-Dependent Anion Channel 1; EC 2.7.1.1/Hexokinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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