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The VC1777-VC1779 proteins are members of a sialic acid-specific subfamily of TRAP transporters (SiaPQM) and constitute the sole route of sialic acid uptake in the human pathogen Vibrio cholerae.
MedLine Citation:
PMID:  22556361     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Sialic acids are nine carbon amino sugars that are present on all mucous membranes and are often used by bacteria as nutrients. In pathogenic Vibrio the genes for sialic acid catabolism are known to be important for host colonization, yet the route for sialic acid uptake is not proven. Vibrio cholerae contains a tripartite ATP independent periplasmic (TRAP) transporter, SiaPQM (VC1777-VC1779) encoded by genes within the Vibrio pathogenicity island-2 (VPI-2), that are adjacent to the sialic acid catabolism genes nanA, nanE and nanK. We demonstrate a correlation of the occurrence of VPI-2 and the ability of Vibrio to grow on the common sialic acid N-acetylneuraminic acid (Neu5Ac) and that a V. cholerae N16961 mutant defective in vc1777, encoding the large membrane protein component of the TRAP transporter, SiaM, is unable to grow on Neu5Ac as the sole carbon source. Using the genome context and known structures of the SiaP protein component of the TRAP transporter, we define a sub-family of Neu5Ac-specific TRAP transporters of which the vc1777-vc1779 genes are the only representatives in V. cholerae. A recent report has suggested that an entirely different TRAP transporter (VC1927-VC1929) is the Neu5Ac transporter in V. cholerae. Bioinformatics and genomic analysis suggests strongly that this is a C4-dicarboxylate-specific TRAP transporter and indeed disruption of vc1929 results in a defect in growth on C4-dicarboxylates but not Neu5Ac. Together these data demonstrate unequivocally that the siaPQM encoded TRAP transporter within VPI-2 is the sole sialic acid transporter in V. cholerae.
Authors:
Nityananda Chowdhury; Jessica Norris; Erin McAlister; Kathy Lau; Gavin H Thomas; E Fidelma Boyd
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-3
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of Delaware;
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