Document Detail


VAMP-2 promotes neurite elongation and SNAP-25A increases neurite sprouting in PC12 cells.
MedLine Citation:
PMID:  10958975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies suggest that the soluble N-ethylmaleimide-sensitive factor attached protein (SNAP) receptor (SNARE)-mediated membrane fusion system is involved in vesicle fusion in the plasma membrane that allows expansion for neurite elongation. There have been several reports analyzing the effects of neurite outgrowth by inhibition of SNAREs. In this study, we took the opposite approach by overexpressing green fluorescent protein (GFP)-fusion SNAREs, including VAMP-2, SNAP-25A, and syntaxin1A, in PC12 cells to investigate the role of SNAREs in the neurite outgrowth of PC12 cells. Neurite outgrowth analysis demonstrated that: (1) GFP-VAMP-2 increased the length of individual neurites, without changing the number of neurites per cell; (2) GFP-SNAP-25A increased the number of neurites per cell, with no change in the length of the individual neurites. In both cases, the total length of neurites per cell was increased; (3) GFP-syntaxin1A resulted in no significant change, either in neurite length, or in the number of neurites per cell. These findings suggest that when overexpressed in PC12 cells, VAMP-2 can promote neurite elongation, while SNAP-25A can stimulate neurite sprouting. On the other hand, overexpression of syntaxin1A neither promotes nor inhibits neurite outgrowth. Thus VAMP-2 and SNAP-25A play different roles in neurite elongation and sprouting.
Authors:
M Shirasu; K Kimura; M Kataoka; M Takahashi; S Okajima; S Kawaguchi; Y Hirasawa; C Ide; A Mizoguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  37     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-15     Completed Date:  2000-09-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  265-75     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Neurobiology, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Surface / genetics,  metabolism*
Green Fluorescent Proteins
Immunoblotting
Luminescent Proteins / genetics
Membrane Proteins / genetics,  metabolism*
Microscopy, Confocal
Microscopy, Immunoelectron
Nerve Tissue Proteins / genetics,  metabolism*
Neurites / physiology*
PC12 Cells
Precipitin Tests
R-SNARE Proteins
Rats
Recombinant Fusion Proteins / genetics,  metabolism
Synaptosomal-Associated Protein 25
Syntaxin 1
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Luminescent Proteins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/R-SNARE Proteins; 0/Recombinant Fusion Proteins; 0/Snap25 protein, rat; 0/Synaptosomal-Associated Protein 25; 0/Syntaxin 1; 147336-22-9/Green Fluorescent Proteins

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