| VAMP-1, VAMP-2, and syntaxin-4 regulate ANP release from cardiac myocytes. | |
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MedLine Citation:
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PMID: 20801128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ANP is a peptide released by cardiac myocytes that regulates blood pressure and natriuresis. However, the molecular mechanisms controlling ANP release from cardiac myocytes are not defined. We now identify three components of the exocytic machinery that regulate ANP release from atrial myocytes. We found that cardiac myocytes express N-ethylmaleimide sensitive factor (NSF), soluble NSF attachment protein (α-SNAP), and SNAP receptors (SNAREs). Additionally we found that specific SNARE molecules, VAMP-1 and VAMP-2, both co-sediment and co-localize with ANP. Also, one SNARE molecule, syntaxin-4, partially co-sediments and partially co-localizes with ANP. Furthermore, these three SNAREs, syntaxin-4 and VAMP-1 and VAMP-2, form a SNARE complex inside cardiac myocytes. Finally, knockdown of VAMP-1, VAMP-2, or syntaxin-4 blocks regulated release of ANP. In contrast, silencing of VAMP-3 did not have an effect on ANP release. Our data suggest that three specific SNAREs regulate cardiac myocyte exocytosis of ANP. Pathways that modify the exocytic machinery may influence natriuresis and blood pressure. |
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Authors:
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Marcella Ferlito; William B Fulton; Mohamed A Zauher; Eduardo Marbán; Charles Steenbergen; Charles J Lowenstein |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-27 |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: 49 ISSN: 1095-8584 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2011-01-18 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: England |
Other Details:
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Languages: eng Pagination: 791-800 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. mferlit1@jhmi.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Atrial Natriuretic Factor / secretion* Cell Separation Endothelins / metabolism Hela Cells Humans Mice Myocytes, Cardiac / secretion* Protein Binding Protein Transport Qa-SNARE Proteins / metabolism* Rats Vesicle-Associated Membrane Protein 1 / metabolism* Vesicle-Associated Membrane Protein 2 / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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P01 HL065608-100007/HL/NHLBI NIH HHS; P01 HL56091/HL/NHLBI NIH HHS; P01 HL65608/HL/NHLBI NIH HHS; P50 HL052315-050003/HL/NHLBI NIH HHS; R01 HL063706-04/HL/NHLBI NIH HHS; R01 HL074061/HL/NHLBI NIH HHS; R01 HL074061-04/HL/NHLBI NIH HHS; R01 HL078635-04/HL/NHLBI NIH HHS; R01 HL63706/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Endothelins; 0/Qa-SNARE Proteins; 0/Vesicle-Associated Membrane Protein 1; 0/Vesicle-Associated Membrane Protein 2; 85637-73-6/Atrial Natriuretic Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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